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首页> 外文期刊>RSC Advances >Preparation, characterization, bioactivity and degradation behavior in vitro of copper-doped calcium polyphosphate as a candidate material for?bone tissue engineering
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Preparation, characterization, bioactivity and degradation behavior in vitro of copper-doped calcium polyphosphate as a candidate material for?bone tissue engineering

机译：掺杂铜的聚磷酸钙作为骨组织工程候选材料的制备，表征，生物活性和体外降解行为

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In this study, copper, as one of the essential trace elements in the human body, was introduced into calcium polyphosphate (CPP) to prepare a novel scaffold in bone tissue engineering: copper-doped calcium polyphosphate (CCPP) scaffolds. This novel scaffold was characterized by XRD, FTIR and SEM. The porosity and mechanical properties of CCPP scaffolds were also investigated. Finally, its bioactivity and degradation behavior in vitro were exploited. The results suggested that low-content copper doping had no significant influence on the structure of CPP. The compressive strength of CCPP with 70% porosity was 5–9 MPa which met the strength requirements of cancellous bone (2–12 MPa). The results of degradation experiments from the weight loss of scaffolds and the release of ions obtained from ICP-OES showed that both the incorporation of copper and acidic metabolites from osteoblasts could promote the degradation of CCPP scaffolds. The amount of Cu2+, Ca2+ and PO43? released from various scaffolds in osteoblast-mediated degradation might be used to elucidate the cytocompatibility of CCPP scaffolds with HUVECs/osteoblasts. MTT assay showed that 0.05% CCPP scaffolds showed a good cytocompatibility with HUVECs/osteoblasts. The ELISA assay showed that 0.05% CCPP scaffolds could promote the secretion of VEGF, ALP and OCN from osteoblasts as well as VEGF from HUVECs, which indicated its good ability to stimulate osteogenesis and angiogenesis. According to the chemical composition and structure of 0.05% CCPP scaffolds, we inferred that 0.05% CCPP scaffolds showed better cytocompatibility and ability to stimulate osteogenesis and angiogenesis due to the synergy effects of copper and the energy produced by the breakage of POP bonds between [PO3?] units in CCPP compared with CPP scaffolds and other Cu-containing biomaterials such as copper-doped hydroxyapatite. The results obtained in our study suggest that 0.05% CCPP scaffold is a promising biomaterial for bone repairing applications.
机译：在这项研究中，将铜作为人体必需的微量元素之一，引入了聚磷酸钙（CPP）中，以制备骨组织工程学中的新型支架：掺杂铜的聚磷酸钙（CCPP）支架。该新型支架通过XRD，FTIR和SEM表征。还研究了CCPP支架的孔隙率和力学性能。最后，研究了其在体外的生物活性和降解行为。结果表明，低含量的铜掺杂对CPP的结构没有显着影响。孔隙率为70％的CCPP的抗压强度为5-9 MPa，满足松质骨的强度要求（2-12 MPa）。支架重量损失和ICP-OES释放离子的降解实验结果表明，铜和酸性代谢产物从成骨细胞中的掺入均可促进CCPP支架的降解。 Cu 2 + ，Ca 2 + 和PO 4 3？ 可能用于阐明CCPP支架与HUVEC /成骨细胞的细胞相容性。 MTT分析显示0.05％CCPP支架与HUVEC /成骨细胞显示出良好的细胞相容性。 ELISA法检测结果表明，0.05％的CCPP支架可以促进成骨细胞VEGF，ALP和OCN的分泌以及HUVECs的VEGF的分泌，这表明其具有良好的刺激成骨和血管生成的能力。根据0.05％CCPP支架的化学组成和结构，我们推断0.05％CCPP支架由于铜的协同作用和P O P断裂产生的能量而显示出更好的细胞相容性以及刺激成骨和血管生成的能力。 CCPP中[PO 3 ？ ]单元之间的键与CPP支架和其他含Cu生物材料相比铜掺杂羟基磷灰石。我们的研究结果表明，0.05％CCPP支架是一种有前途的生物材料，可用于骨修复。

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