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首页> 外文期刊>RSC Advances >Preparation, characterization, bioactivity and degradation behavior in vitro of copper-doped calcium polyphosphate as a candidate material for bone tissue engineering
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Preparation, characterization, bioactivity and degradation behavior in vitro of copper-doped calcium polyphosphate as a candidate material for bone tissue engineering

机译：铜掺杂钙多磷酸钙体外的制备，表征，生物活性和降解行为作为骨组织工程候选材料

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摘要

In this study, copper, as one of the essential trace elements in the human body, was introduced into calcium polyphosphate (CPP) to prepare a novel scaffold in bone tissue engineering: copper-doped calcium polyphosphate (CCPP) scaffolds. This novel scaffold was characterized by XRD, FTIR and SEM. The porosity and mechanical properties of CCPP scaffolds were also investigated. Finally, its bioactivity and degradation behavior in vitro were exploited. The results suggested that low-content copper doping had no significant influence on the structure of CPP. The compressive strength of CCPP with 70% porosity was 5-9 MPa which met the strength requirements of cancellous bone (2-12 MPa). The results of degradation experiments from the weight loss of scaffolds and the release of ions obtained from ICP-OES showed that both the incorporation of copper and acidic metabolites from osteoblasts could promote the degradation of CCPP scaffolds. The amount of Cu2+, Ca2+ and PO43- released from various scaffolds in osteoblast-mediated degradation might be used to elucidate the cytocompatibility of CCPP scaffolds with HUVECs/osteoblasts. MTT assay showed that 0.05% CCPP scaffolds showed a good cytocompatibility with HUVECs/osteoblasts. The ELISA assay showed that 0.05% CCPP scaffolds could promote the secretion of VEGF, ALP and OCN from osteoblasts as well as VEGF from HUVECs, which indicated its good ability to stimulate osteogenesis and angiogenesis. According to the chemical composition and structure of 0.05% CCPP scaffolds, we inferred that 0.05% CCPP scaffolds showed better cytocompatibility and ability to stimulate osteogenesis and angiogenesis due to the synergy effects of copper and the energy produced by the breakage of POP bonds between [PO3-] units in CCPP compared with CPP scaffolds and other Cu-containing biomaterials such as copper-doped hydroxyapatite. The results obtained in our study suggest that 0.05% CCPP scaffold is a promising biomaterial for bone repairing applications.
机译：在该研究中，将铜作为人体中的必需微量元素之一被引入多磷酸钙（CPP）中，以制备骨组织工程中的新型支架：铜掺杂的多磷酸钙（CCPP）支架。这种新型支架的特征在于XRD，FTIR和SEM。还研究了CCPP支架的孔隙率和机械性能。最后，利用其体外生物活性和降解行为。结果表明，低含量的铜掺杂对CPP的结构没有显着影响。具有70％孔隙率的CCPP的抗压强度为5-9MPa，符合松质骨（2-12MPa）的强度要求。从ICP-OES获得的重量损失和从ICP-OE获得的离子释放的降解实验结果表明，来自成骨细胞的铜和酸性代谢物的掺入可以促进CCPP支架的降解。从成骨细胞介导的降解中的各种支架中释放的Cu 2 +，Ca2 +和PO43的量可用于阐明CCPP支架与HUVECS /骨盆的细胞相容性。 MTT测定表明，0.05％CCPP支架显示出与Huvecs /骨液细胞的良好的细胞组成性。 ELISA测定表明，0.05％CCPP支架可以促进VEGF，ALP和OCN的分泌来自成骨细胞以及HUVECS的VEGF，这表明其刺激成骨和血管生成的良好能力。根据0.05％CCPP支架的化学成分和结构，我们推断出0.05％CCPP支架表明由于铜的协同效应和由P O P破损产生的能量而刺激骨发生和血管生成的能力更好与CCPP中的[PO3-]单元之间的键合与CPP支架和其他含Cu的生物材料如铜掺杂的羟基磷灰石相比。在我们的研究中获得的结果表明，0.05％CCPP支架是骨修复应用的有希望的生物材料。

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  • 来源
    《RSC Advances》 |2017年第67期|共13页
  • 作者

    Guo Chengrui; Li Li; Li Shuangshuang; Wang Yaping; Yu Xixun;

  • 作者单位

    Sichuan Univ Coll Polymer Sci &

    Engn Chengdu 610065 Sichuan Peoples R China;

    452 Hosp Chinese PLA Dept Oncol Chengdu 610021 Sichuan Peoples R China;

    Sichuan Univ Coll Polymer Sci &

    Engn Chengdu 610065 Sichuan Peoples R China;

    Sichuan Univ Coll Polymer Sci &

    Engn Chengdu 610065 Sichuan Peoples R China;

    Sichuan Univ Coll Polymer Sci &

    Engn Chengdu 610065 Sichuan Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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