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Hyaluronic acid-based carbon dots for efficient gene delivery and cell imaging

机译：基于透明质酸的碳点，可高效传递基因并进行细胞成像

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摘要

A strategy that uses hyaluronic acid (HA) as a carbon source and polyethylenimine (PEI) as a passivant to construct carbon dots (HA-Cds) was proposed. The synthetic method is simple and green and no additive was required. These carbon dots could emit strong blue fluorescence under UV light. FT-IR and 1H NMR spectra confirmed that part of characteristic residues of HA and PEI remained in the HA-Cds structure. These materials had much lower cytotoxicity than PEI and high serum tolerance. Up to 50 times higher transfection efficiency than that of PEI was obtained in the presence of 10% serum. BSA protein adsorption, flow cytometry, and confocal microscopy assays also supported their good performance with serum. Furthermore, as multifunctional materials, HA-Cds had good intracellular imaging ability and displayed tunable fluorescence emission under varying excitation wavelengths. An HA competition assay showed that they may have target cell imaging ability in CD44 overexpressed cells. These materials with fluorescence activity also facilitated co-localization experiments by CLSM, which revealed that the DNA cargo could be effectively released into the cytosol, leading to effective gene transfection. These properties make the carbon dots promising candidates for in vivo diagnosis and gene therapy.
机译：提出了使用透明质酸（HA）作为碳源和聚乙烯亚胺（PEI）作为钝化剂来构建碳点（HA-Cds）的策略。合成方法简单，绿色，不需要添加剂。这些碳点在紫外线下会发出强烈的蓝色荧光。 FT-IR和 1 1 H NMR光谱证实，HA和PEI的部分特征残基保留在HA-Cds结构中。这些材料的细胞毒性远低于PEI，并且具有较高的血清耐受性。在存在10％血清的情况下，获得的转染效率比PEI高50倍。 BSA蛋白吸附，流式细胞术和共聚焦显微镜分析也支持它们在血清中的良好性能。此外，作为多功能材料，HA-Cds具有良好的细胞内成像能力，并在变化的激发波长下显示出可调的荧光发射。 HA竞争分析表明，它们可能在CD44过表达的细胞中具有靶细胞显像能力。这些具有荧光活性的材料还促进了CLSM的共定位实验，这表明DNA货物可以有效地释放到细胞质中，导致有效的基因转染。这些特性使碳点有望成为体内诊断和基因治疗的候选者。

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