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Hyaluronic acid-based carbon dots for efficient gene delivery and cell imaging

机译：基于透明质酸的碳点，用于有效基因递送和细胞成像

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摘要

A strategy that uses hyaluronic acid (HA) as a carbon source and polyethylenimine (PEI) as a passivant to construct carbon dots (HA-Cds) was proposed. The synthetic method is simple and green and no additive was required. These carbon dots could emit strong blue fluorescence under UV light. FT-IR and H-1 NMR spectra confirmed that part of characteristic residues of HA and PEI remained in the HA-Cds structure. These materials had much lower cytotoxicity than PEI and high serum tolerance. Up to 50 times higher transfection efficiency than that of PEI was obtained in the presence of 10% serum. BSA protein adsorption, flow cytometry, and confocal microscopy assays also supported their good performance with serum. Furthermore, as multifunctional materials, HA-Cds had good intracellular imaging ability and displayed tunable fluorescence emission under varying excitation wavelengths. An HA competition assay showed that they may have target cell imaging ability in CD44 overexpressed cells. These materials with fluorescence activity also facilitated co-localization experiments by CLSM, which revealed that the DNA cargo could be effectively released into the cytosol, leading to effective gene transfection. These properties make the carbon dots promising candidates for in vivo diagnosis and gene therapy.
机译：提出了一种用透明质酸（HA）作为碳源和聚乙烯（PEI）作为构建碳点（HA-CD）的碳源和聚乙烯酸酯（PEI）的策略。合成方法简单且绿色，不需要添加剂。这些碳点可以在UV光下发出强大的蓝色荧光。 FT-IR和H-1 NMR光谱证实，HA和PEI的一部分特征残留物保留在HA-CDS结构中。这些材料具有比PEI和高血清耐受性更低的细胞毒性。在10％血清存在下获得的转染效率高达50倍的转染效率。 BSA蛋白吸附，流式细胞术和共聚焦显微镜测定也支持它们的血清性能。此外，作为多官能材料，HA-CD具有良好的细胞内成像能力，并在不同的激发波长下显示可调荧光发射。 HA竞争测定表明，在CD44过表达细胞中，它们可能具有靶细胞成像能力。这些具有荧光活性的这些材料还通过CLSM促进了共定位实验，这揭示了DNA货物可以有效地释放到细胞质中，导致有效的基因转染。这些特性使碳点具有用于体内诊断和基因治疗的候选者。

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  • 来源
    《RSC Advances》 |2017年第25期|共12页
  • 作者

    Wang Hai-Jiao; Zhang Ji; Liu Yan-Hong; Luo Tian-Ying; He Xi; Yu Xiao-Qi;

  • 作者单位

    Sichuan Univ Key Lab Green Chem &

    Technol Minist Educ Coll Chem Chengdu 610064 Peoples R China;

    Sichuan Univ Key Lab Green Chem &

    Technol Minist Educ Coll Chem Chengdu 610064 Peoples R China;

    Sichuan Univ Key Lab Green Chem &

    Technol Minist Educ Coll Chem Chengdu 610064 Peoples R China;

    Sichuan Univ Key Lab Green Chem &

    Technol Minist Educ Coll Chem Chengdu 610064 Peoples R China;

    Sichuan Univ Key Lab Green Chem &

    Technol Minist Educ Coll Chem Chengdu 610064 Peoples R China;

    Sichuan Univ Key Lab Green Chem &

    Technol Minist Educ Coll Chem Chengdu 610064 Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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