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首页> 外文期刊>RSC Advances >Combined effect of tris(2-chloroethyl)phosphate and benzo (a) pyrene on the release of IL-6 and IL-8 from HepG2 cells via the EGFR-ERK1/2 signaling pathway
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Combined effect of tris(2-chloroethyl)phosphate and benzo (a) pyrene on the release of IL-6 and IL-8 from HepG2 cells via the EGFR-ERK1/2 signaling pathway

机译：磷酸三（2-氯乙基）酯和苯并（a）pyr通过EGFR-ERK1 / 2信号通路对HepG2细胞IL-6和IL-8释放的联合作用

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摘要

Tris(2-chloroethyl)phosphate (TCEP) and benzo (a) pyrene (BaP) coexist in the environment. Humans are exposed to them via multiple routes every day. Each of them induces hepatotoxicity, which may increase their risk to human health. However, the mechanism underlying the combined toxicity of both compounds in vitro is still unclear. The present study aimed to investigate the molecular mechanism underlying the inflammatory response in the cotreatment of HepG2 cells with TCEP and BaP. The cell viability, and the expression of interleukin (IL)-6 and IL-8 at the mRNA and protein levels were measured in HepG2 cells. The results indicated that TCEP plus BaP decreased HepG2 cell viability, and up-regulated the expression of IL-6 and IL-8 at the mRNA and protein levels. Additionally, the inhibitors of EGFR (AG1478), ERK1/2 (U0126) and p38 MAPK (SB203580) displayed anti-inflammatory properties in the inflammatory response elicited by TCEP plus BaP. The activation of ERK1/2, but not p38 MAPK was inhibited by AG1478. These results indicated that TCEP plus BaP may induce an inflammatory response in HepG2 cells by the activation of the EGFR-ERK1/2 signaling pathway.
机译：三（2-氯乙基）磷酸酯（TCEP）和苯并（a）pyr（BaP）共存于环境中。每天，人类会通过多种途径 与他们接触。他们每个人诱发肝毒性，这可能会增加其对人体健康的风险。然而，这两种化合物在体外 的联合毒性的潜在机制仍不清楚。本研究旨在研究与TCEP和BaP共同治疗HepG2细胞中炎症反应的分子机制。在HepG2细胞中测量细胞活力以及白细胞介素（IL）-6和IL-8在mRNA和蛋白质水平的表达。结果表明，TCEP加BaP降低了HepG2细胞的活力，并在mRNA和蛋白质水平上调了IL-6和IL-8的表达。另外，EGFR（AG1478），ERK1 / 2（U0126）和p38 MAPK（SB203580）的抑制剂在TCEP加BaP引起的炎症反应中显示出抗炎特性。 AG1478抑制ERK1 / 2的激活，但不抑制p38 MAPK的激活。这些结果表明，TCEP加BaP可能通过激活EGFR-ERK1 / 2信号通路在HepG2细胞中诱导炎症反应。

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