Combined effect of tris(2-chloroethyl) phosphate and benzo (a) pyrene on the release of IL-6 and IL-8 from HepG2 cells via the EGFR-ERK1/2 signaling pathway

Zhang Youjian; Zhang Wenjuan; Hou Jian; Wang Xian; Zheng Hongyan; Xiong Wei; Yuan Jing

首页> 外文期刊>RSC Advances >Combined effect of tris(2-chloroethyl) phosphate and benzo (a) pyrene on the release of IL-6 and IL-8 from HepG2 cells via the EGFR-ERK1/2 signaling pathway

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Combined effect of tris(2-chloroethyl) phosphate and benzo (a) pyrene on the release of IL-6 and IL-8 from HepG2 cells via the EGFR-ERK1/2 signaling pathway

机译：Tris（2-氯乙基）磷酸盐和苯并（a）芘对通过EGFR-ERK1 / 2信号通路从HepG2细胞释放IL-6和IL-8的组合作用

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Tris(2-chloroethyl) phosphate (TCEP) and benzo (a) pyrene (BaP) coexist in the environment. Humans are exposed to them via multiple routes every day. Each of them induces hepatotoxicity, which may increase their risk to human health. However, the mechanism underlying the combined toxicity of both compounds in vitro is still unclear. The present study aimed to investigate the molecular mechanism underlying the inflammatory response in the cotreatment of HepG2 cells with TCEP and BaP. The cell viability, and the expression of interleukin (IL)-6 and IL-8 at the mRNA and protein levels were measured in HepG2 cells. The results indicated that TCEP plus BaP decreased HepG2 cell viability, and up-regulated the expression of IL-6 and IL-8 at the mRNA and protein levels. Additionally, the inhibitors of EGFR (AG1478), ERK1/2 (U0126) and p38 MAPK (SB203580) displayed anti-inflammatory properties in the inflammatory response elicited by TCEP plus BaP. The activation of ERK1/2, but not p38 MAPK was inhibited by AG1478. These results indicated that TCEP plus BaP may induce an inflammatory response in HepG2 cells by the activation of the EGFR-ERK1/2 signaling pathway.

机译：TRIS（2-氯乙基）磷酸盐（TCEP）和苯并（A）芘（BAP）在环境中共存。人类每天都会通过多条路线暴露在他们身边。他们中的每一个都会诱导肝毒性，这可能会增加他们对人类健康的风险。然而，在体外两种化合物的组合毒性的基础仍然不清楚。本研究旨在探讨HepG2细胞与TCEP和BAP的CoTreative act的分子机制。在HepG2细胞中测量细胞活力和白细胞介素（IL）-6和IL-8的表达。结果表明，TCEP Plus BAP降低了HepG2细胞活力，上调了在mRNA和蛋白质水平下IL-6和IL-8的表达。另外，EGFR（AG1478），ERK1 / 2（U0126）和P38MAPK（SB203580）的抑制剂显示在由TCEP加阵切引发的炎症反应中的抗炎性质。 AG1478抑制了ERK1 / 2的激活，但不是P38 MAPK。这些结果表明，TCEP Plus Bap通过激活EGFR-ERK1 / 2信号通路可以在HepG2细胞中诱导炎症反应。

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《RSC Advances》 |2017年第85期|共10页
作者
Zhang Youjian; Zhang Wenjuan; Hou Jian; Wang Xian; Zheng Hongyan; Xiong Wei; Yuan Jing;
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Huazhong Univ Sci &

Technol Tongji Med Coll Dept Occupat &

Environm Hlth Wuhan 430030 Hubei Peoples R China;

Huazhong Univ Sci &

Technol Tongji Med Coll Dept Occupat &

Environm Hlth Wuhan 430030 Hubei Peoples R China;

Huazhong Univ Sci &

Technol Tongji Med Coll Dept Occupat &

Environm Hlth Wuhan 430030 Hubei Peoples R China;

Huazhong Univ Sci &

Technol Tongji Med Coll Dept Occupat &

Environm Hlth Wuhan 430030 Hubei Peoples R China;

Huazhong Univ Sci &

Technol Tongji Med Coll Dept Occupat &

Environm Hlth Wuhan 430030 Hubei Peoples R China;

Huazhong Univ Sci &

Technol Tongji Med Coll Dept Occupat &

Environm Hlth Wuhan 430030 Hubei Peoples R China;

Huazhong Univ Sci &

Technol Tongji Med Coll Dept Occupat &

Environm Hlth Wuhan 430030 Hubei Peoples R China;

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1. Combined effect of tris(2-chloroethyl) phosphate and benzo (a) pyrene on the release of IL-6 and IL-8 from HepG2 cells via the EGFR-ERK1/2 signaling pathway [J] . Zhang Youjian, Zhang Wenjuan, Hou Jian, RSC Advances . 2017,第85期

机译：Tris（2-氯乙基）磷酸盐和苯并（a）芘对通过EGFR-ERK1 / 2信号通路从HepG2细胞释放IL-6和IL-8的组合作用
2. Glucose 6-phosphate dehydrogenase knockdown enhances IL-8 expression in HepG2 cells via oxidative stress and NF-κB signaling pathway [J] . Hung-Chi Yang, Mei-Ling Cheng, Yi-Syuan Hua, Journal of inflammation. . 2015,第S1期

机译：6-磷酸葡萄糖脱氢酶的抑制通过氧化应激和NF-κB信号通路增强HepG2细胞中IL-8的表达
3. LPS Synergizing With Poly-L-arginine Activate NCI-H292 Cells to Release IL-6 and IL-8 via JNK Signaling Pathway [J] . Xiao Yun Fan, Bing Chen, Yu Wen Liu, Chest . 2016,第4期

机译：与聚L-精氨酸协同作用的LPS激活NCI-H292细胞通过JNK信号通路释放IL-6和IL-8
4. Construction of signaling pathways and identification of drug effects on the liver cancer cell HepG2 [C] . Alexopoulos Leonidas G., Melas Ioannis N., Chairakaki Aikaterini D., 32nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society . 2010

机译：肝癌细胞HepG2信号通路的构建及药物作用的鉴定
5. Benzo(a)pyrene-mediated effects on cellular adhesion and signal transduction pathways in endometrial cancer. [D] . McGarry, Michelle Ann. 2001

机译：苯并（a）-介导的对子宫内膜癌中细胞粘附和信号转导途径的影响。
6. Tris(2-chloroethyl) Phosphate (TCEP) Elicits Hepatotoxicity by Activating Human Cancer Pathway Genes in HepG2 Cells [O] . Abdullah M. Al-Salem, Quaiser Saquib, Maqsood A. Siddiqui, 2020

机译：Tris（2-氯乙基）磷酸盐（TCEP）通过在HepG2细胞中激活人癌途径基因来引发肝毒性
7. Combined effect of tris(2-chloroethyl)phosphate and benzo (a) pyrene on the release of IL-6 and IL-8 from HepG2 cells via the EGFR-ERK1/2 signaling pathway [O] . Youjian Zhang, Wenjuan Zhang, Jian Hou, 2017

机译：Tris（2-氯乙基）磷酸盐和苯并（a）芘对通过EGFR-ERK1 / 2信号通路从HepG2细胞释放IL-6和IL-8的组合作用

Combined effect of tris(2-chloroethyl) phosphate and benzo (a) pyrene on the release of IL-6 and IL-8 from HepG2 cells via the EGFR-ERK1/2 signaling pathway

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