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One-step fabrication of a tunable nanofibrous well insert via electrolyte-assisted electrospinning

机译：通过电解质辅助电纺丝一步一步制备可调谐纳米纤维孔插入物

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The integration of the Transwell? assay with an electrospun nanofiber membrane shows a significant potential in chemotactic assays and co-culture models, but the complicated integration processes often limit its utilization. Here, we present a one-step fabrication process of a nanofibrous well insert by adopting electrolyte-assisted electrospinning, named ELES. The utilization of ELES, which introduced the electrolyte solution as a temporal collector, enabled the facilitation of not only the fabrication of a free-standing electrospun polycaprolactone (PCL) nanofiber on the bottom opening of a well insert wall but also the spontaneous integration between the nanofiber membrane and the well insert wall in a one-step process. The versatility of this approach was demonstrated by modulating the diameter of PCL nanofibers and thickness of the membrane. The indentation test revealed stable integration between the membrane and the well insert wall. The fabricated nanofibrous well inserts were confirmed as an in vitro cell culture platform with promising cell culture results of mouse brain endothelial cell line (bEnd.3).
机译：整合Transwell吗？静电纺丝纳米纤维膜的化学分析在趋化分析和共培养模型中显示出巨大的潜力，但是复杂的整合过程常常限制了其利用。在这里，我们介绍了一种通过采用电解质辅助电纺丝（称为ELES）的纳米纤维孔插入物的一步制造工艺。 ELES的使用将电解质溶液作为临时的集电极引入，不仅有助于在井壁壁的底部开口上制造自立式电纺聚己内酯（PCL）纳米纤维，而且还促进了两者之间的自发整合。纳米纤维膜和孔插入壁的一步式过程。通过调节PCL纳米纤维的直径和膜的厚度证明了这种方法的多功能性。压痕测试显示了膜和孔插入壁之间的稳定整合。所制备的纳米纤维孔插入物被证实是一种体外细胞培养平台，具有小鼠脑内皮细胞系（bEnd.3）良好的细胞培养结果。

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