Efficient synthesis of ponasterone A by recombinant Escherichia coli harboring the glycosyltransferase GTBP1 with in situ product removal

Bingfeng Li; Xuejun He; Bo Fan; Jianlin Chu; Bingfang He

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Efficient synthesis of ponasterone A by recombinant Escherichia coli harboring the glycosyltransferase GT_BP1 with in situ product removal

机译：带有糖基转移酶GT _{BP1 的重组大肠杆菌的原位产物去除可有效合成ponasterone A}

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A glycosyltransferase GT_BP1 from Bacillus pumilus BF1 was isolated. Efficient production of ponasterone A was achieved by the recombinant E. coli/gt_BP1 in a biphasic system with a molar yield of 92.7%. This in situ product removal provided the “driving force” for shifting the reaction equilibrium towards the synthesis of the product. 展开▼

机译：分离了短小芽孢杆菌BF1中的糖基转移酶GT _{BP1 。重组 E可实现高效生产ponasteroneA。双相系统中的大肠杆菌 / gt _{BP1 ，摩尔产率为92.7％。这种“原位”产物的去除提供了将反应平衡向产物合成转移的“推动力”。}} 展开▼

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来源
《RSC Advances》 |2017年第37期|共3页

作者
Bingfeng Li; Xuejun He; Bo Fan; Jianlin Chu; Bingfang He;
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中图分类化学;

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机译：在具有七个类胡萝卜素生物合成基因（包括）的重组大肠杆菌表达系统中生产钙黄素3'-β-D-葡萄糖苷，玉米黄质3,3'-β-D-二葡萄糖苷和Nostoxanthin。 crtX 和 crtG 。

2. High-flux isobutanol production using engineered Escherichia coli: A bioreactor study with in situ product removal [J] . Baez A., Cho K.-M., Liao J.C. Applied Microbiology and Biotechnology . 2011,第5期

机译：使用工程化大肠杆菌生产高通量异丁醇：原位去除产物的生物反应器研究

3. High-flux isobutanol production using engineered Escherichia coli: a bioreactor study with in situ product removal [J] . Antonino Baez, Kwang-Myung Cho, James C. Liao Applied Microbiology and Biotechnology . 2011,第5期

机译：使用工程化大肠杆菌生产高通量异丁醇：去除原位产物的生物反应器研究

4. Asymmetric synthesis of ethyl (R)-4-chloro-3-hydroxybutanoate with a two recombinant Escherichia coli system [C] . Ke-Ju Jing, Wen-Ting Ban International Conference on Manufacturing Science and Engineering . 2013

机译：用两种重组大肠杆菌系统的乙基（R）-4-氯-3-羟基苯甲酸酯的不对称合成

5. Recombinant vaccines against infectious hematopoietic necrosis virus: Bacterial systems for vaccine production and delivery (Oncorhynchus mykiss, Caulobacter crescentus, Escherichia coli, Yersinia ruckeri). [D] . Simon, Benjamin E. 2002

机译：抗传染性造血坏死病毒的重组疫苗：用于疫苗生产和递送的细菌系统（Oncorhynchus mykiss，Caulobacter crescentus，大肠杆菌，Yersinia ruckeri）。

6. High-flux isobutanol production using engineered Escherichia coli: a bioreactor study with in situ product removal [O] . Antonino Baez, Kwang-Myung Cho, James C. Liao -1

机译：使用工程化大肠杆菌生产高通量异丁醇：去除原位产物的生物反应器研究

7. High-flux isobutanol production using engineered Escherichia coli: a bioreactor study with in situ product removal [O] . Antonino Baez, Kwang-Myung Cho, James C. Liao 2011

机译：使用工程化大肠杆菌生产高通量异丁醇：去除原位产物的生物反应器研究

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2. DNA FRAGMENT HC 280 PREPARED BY CHEMICAL SYNTHESIS, RECOMBINANT POLYPEPTIDE HC 280 AT MOLECULAR MASS 120-130 KDA SHOWING CAPABILITY TO BIND ANTIBODIES TO GENE-ENCODING PROTEIN NS4 OF HEPATITIS C VIRUS PREPARED AT CULTIVATION OF THE STRAIN OF BACTERIUM ESCHERICHIA COLI - STRAIN OF BACTERIUM ESCHERICHIA COLI - A PRODUCER OF RECOMBINANT POLYPEPTIDE HC 280 SHOWING CAPABILITY TO BIND ANTIBODIES TO GENE-ENCODING PROTEIN NS4 OF HEPATITIS C VIRUS [P] . 外国专利： RU2073718C1 . 1997-02-20

机译：化学合成制备的DNA片段HC 280，分子质量为120-130 KDA的重组多肽HC 280，具有结合抗原的能力，可将编码大肠杆菌的大肠杆菌NS4病毒的基因编码到大肠杆菌中。 COLI-重组多肽HC 280的生产商，具有对肝炎病毒C基因编码蛋白NS4进行抗原结合的能力

3. RECOMBINANT PLASMID DNA pPA-OPRF-ETA, WHICH CODES SYNTHESIS OF HYBRID RECOMBINANT PROTEIN, WHICH INCLUDES AMINO-ACID SEQUENCES OF PROTEIN F OF OUTER MEMBRANE AND EXOTOXIN A PSEUDOMONAS aeruginosa, DEFORMATION OF ESCHERICHIA coli PA-OPRF-ETA - PRODUCER OF HYBRID RECOMBINANT PROTEIN, WHICH INCLUDES AMINO-ACID SEQUENCES OF PROTEIN F OF OUTER MEMBRANE AND EXOTOXIN A PSEUDOMONAS aeruginosa, AND METHOD FOR PRODUCTION OF HYBRID RECOMBINANT PROTEIN, WHICH INCLUDES AMINO-ACID SEQUENCES OF PROTEIN F OF OUTER MEMBRANE AND EXOTOXIN A PSEUDOMONAS aeruginosa [P] . 外国专利： RU2012139928A . 2014-03-27

机译：重组质体DNA pPA-OPRF-ETA，可合成杂种重组蛋白，包括外膜蛋白F的氨基酸序列和铜绿假单胞菌的外毒素，重组大肠杆菌-胰蛋白酶原-蛋白酶。，包括外膜蛋白F和外膜毒素铜绿假单胞菌的氨基酸序列，以及生产杂交重组蛋白的方法，其中包括外膜蛋白F和蛋白质膜外膜蛋白的氨基酸序列

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Efficient synthesis of ponasterone A by recombinant Escherichia coli harboring the glycosyltransferase GTBP1 with in situ product removal

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Efficient synthesis of ponasterone A by recombinant Escherichia coli harboring the glycosyltransferase GT_BP1 with in situ product removal