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首页> 外文期刊>RSC Advances >Graphene/polyaniline electrodeposited needle trap device for the determination of volatile organic compounds in human exhaled breath vapor and A549 cell
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Graphene/polyaniline electrodeposited needle trap device for the determination of volatile organic compounds in human exhaled breath vapor and A549 cell

机译：石墨烯/聚苯胺电沉积针阱装置测定人呼出的蒸气和A549细胞中的挥发性有机化合物

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In this work, a graphene/polyaniline (G/PANI) electrodeposited coating was introduced as a novel extraction phase of needle trap microextraction (NTME). The needle trap coating was prepared by in situ electrodepositing G/PANI on the inner surface of a 21-gauge stainless steel needle, and utilized for the dynamic extraction of volatile organic compounds (VOCs) from human exhaled breath vapor (EBV) and headspace gas in cell lines. The coating exhibited enhanced mechanical stability, long lifespan, large specific surface area and good biocompatibility. The parameters for the extraction and analysis of analytes including extraction mode, sampling temperature, sampling speed, desorption parameters (temperature and time) were evaluated and optimized. The extraction performance of the needle trap was also compared with three commercially available SPME fibers. Low detection limits (0.002–0.003 μg L?1) and good linearity (R2 ≥ 0.9905) were obtained for the analysis of eight VOCs. The developed needle trap microextraction-gas chromatography-mass spectrometry (NTME-GC-MS) method has been successfully applied to the analysis of VOCs from human exhaled breath and lung cancer cell lines (A549) with good recovery (70–120%) and acceptable reproducibility (relative standard deviation: intra-day 4.8–15.3%, inter-day, 4.2–14.3%). The proposed method is simple, sensitive and environmentally friendly, it provides a new possibility for the determination of VOCs of exhaled breath both in vivo and in vitro.
机译：在这项工作中，引入了石墨烯/聚苯胺（G / PANI）电沉积涂层，作为针阱微萃取（NTME）的新型萃取阶段。通过将G / PANI电沉积在21号不锈钢针的内表面上来制备针阱涂层，并用于从呼出气中动态提取挥发性有机化合物（VOC）。细胞系中的蒸气（EBV）和顶空气体。该涂层表现出增强的机械稳定性，长寿命，大比表面积和良好的生物相容性。评估和优化了用于分析物提取和分析的参数，包括提取模式，采样温度，采样速度，解吸参数（温度和时间）。还将捕针器的提取性能与三种市售SPME纤维进行了比较。低检测限（0.002-0.003μgL ？1 ）和良好的线性度（ R 2 < / small>≥0.9905）用于分析8种VOC。发达的针阱微萃取-气相色谱-质谱（NTME-GC-MS）方法已成功用于人呼出气和肺癌细胞系（A549）中VOC的分析，回收率良好（70–120％），并且可接受的重复性（相对标准偏差：日内4.8–15.3％，日间4.2-14.3％）。该方法简便，灵敏，环境友好，为测定体内和体外的呼气中挥发性有机化合物提供了新的可能。

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