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Microbial oil production by Mortierella isabellina from corn stover under different pretreatments

机译:不同预处理条件下玉米秸秆伊莎贝拉菌的微生物产油

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Mixed culture of Trichoderma reesei and Aspergillus niger was employed to accomplish on-site cellulase production where cellulases were applied directly to the enzymatic hydrolysis of pretreated corn stover. We comprehensively compared the five pretreatments including sodium hydroxide, steam explosion, aqueous ammonia, lime and diluted sulfuric acid in the enzymatic hydrolysis and then in the whole processes from corn stover to single cell oil (SCO). The results were not completely but roughly the same. However, it is conclusive that sodium hydroxide pretreatment was the best one. The process with sodium hydroxide pretreatment could produce 23.5 g dry cell biomass harboring 13.7 g single cell oil (SCO) from 146.2 g corn stover. The total yields of cell biomass and SCO were 0.161 and 0.094 g g?1 corn stover, respectively. This process was proven the most efficient. Through this work, we established efficient SCO production process from corn stover.
机译:使用里氏木霉和黑曲霉的混合培养物完成现场纤维素酶的生产,将纤维素酶直接用于预处理的玉米秸秆的酶促水解。我们综合比较了酶解水解以及从玉米秸秆到单细胞油(SCO)的整个过程中的五个预处理,包括氢氧化钠,蒸汽爆炸,氨水,石灰和稀硫酸。结果并不完全相同,但大致相同。但是,结论是氢氧化钠预处理是最好的方法。氢氧化钠预处理可从146.2克玉米秸秆中生产23.5克干细胞生物质,其中包含13.7克单细胞油(SCO)。细胞生物量和SCO的总产量分别为0.161和0.094 g g ?1 玉米秸秆。事实证明,此过程最有效。通过这项工作,我们从玉米秸秆建立了有效的SCO生产流程。

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