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首页> 外文期刊>RSC Advances >The solid-phase extraction of α-chymotrypsin based on a novel porous polymeric dianionic ionic liquid-coated magnetic material
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The solid-phase extraction of α-chymotrypsin based on a novel porous polymeric dianionic ionic liquid-coated magnetic material

机译：基于新型多孔聚合双阴离子液体包覆磁性材料的α-胰凝乳蛋白酶固相萃取

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摘要

A novel magnetic solid-phase extraction (MSPE) method based on carboxymethylcellulose sodium modified Fe3O4 nanocomposite coated with porous polymeric dianionic ionic liquid (Fe3O4@CMC@PPDIL) was proposed and applied to extracting α-chymotrypsin. The synthesized Fe3O4@CMC@PPDIL was characterized by vibrating sample magnetometry (VSM), thermo-gravimetric analysis (TGA), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectrometry (FT-IR) and zeta potential measurement. Furthermore, the influence of a series of factors, such as extraction time and temperature, initial α-chymotrypsin concentration, pH value and ionic strength, was systematically investigated. Under the optimal extraction conditions, the extraction capacity for α-chymotrypsin could reach up to 122.91 mg g?1, and the activity of α-chymotrypsin is well maintained after extraction. In addition, the proposed MSPE method provides satisfactory results in real sample analysis.
机译：羧甲基纤维素钠修饰的Fe 3 O 4 纳米复合涂层包覆的磁性固相萃取新方法提出了一种多孔聚合的双阴离子溶液（Fe 3 O 4 @ CMC @ PPDIL）并用于萃取α-胰凝乳蛋白酶。合成的Fe 3 O 4 @ CMC @ PPDIL的特征是通过振动样品磁强计（VSM），热-重量分析（TGA），透射电子显微镜（TEM），X射线衍射（XRD），傅里叶变换红外光谱（FT-IR）和Zeta电位测量。此外，系统地研究了萃取时间和温度，初始α-胰凝乳蛋白酶浓度，pH值和离子强度等一系列因素的影响。在最佳提取条件下，α-胰凝乳蛋白酶的提取量可达122.91 mg g ？1 ，提取后α-胰凝乳蛋白酶的活性得到很好的维持。另外，所提出的MSPE方法在实际样品分析中提供了令人满意的结果。

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