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首页> 外文期刊>RSC Advances >Tantalum nitride coatings prepared by magnetron sputtering to improve the bioactivity and osteogenic activity for titanium alloy implants
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Tantalum nitride coatings prepared by magnetron sputtering to improve the bioactivity and osteogenic activity for titanium alloy implants

机译：通过磁控溅射制备的氮化钽涂层可改善钛合金植入物的生物活性和成骨活性

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摘要

A good orthopedic load-bearing implant should have both excellent mechanical properties and biological activity. In order to improve the biological activity of Ti6Al4V-based implants, tantalum nitride (TaN) films were deposited on the surface of Ti6Al4V alloy and Si(100) substrates by magnetron sputtering. Characterized by XRD and XPS, it was proved that the uniform cubic NaCl-structure TaN films were successfully prepared on the Ti6Al4V alloy substrate. The TaN film showed higher hardness (19.7 GPa) and elastic modulus (254.4 GPa) compared with HA coatings and Ti6Al4V alloys. We evaluated cytocompatibility and osteogenesis activity of the TaN coatings using rabbit bone marrow stromal cells (BMSCs) cultured on the coated and uncoated samples. The biocompatibility of the films and their effect on cell proliferation were examined by live/dead cell staining and CCK-8 assay. SEM and cytoskeleton fluorescent staining were used to observe the cell adhesion and morphology on different samples. The effect of the film on osteoblastic activity was assessed by calcium nodule staining and alkaline phosphatase quantitative assays. We demonstrate that TaN film can enhance the adhesion, proliferation, and differentiation of BMSCs. These results suggest that TaN film prepared by magnetron sputtering has great potential to be applied in orthopedics.
机译：良好的骨科负重植入物应具有出色的机械性能和生物学活性。为了提高基于Ti6Al4V的植入物的生物活性，通过磁控溅射将氮化钽（TaN）膜沉积在Ti6Al4V合金和Si（100）衬底的表面上。通过XRD和XPS表征，证明在Ti6Al4V合金基底上成功制备了均匀的立方NaCl结构的TaN薄膜。与HA涂层和Ti6Al4V合金相比，TaN膜显示出更高的硬度（19.7 GPa）和弹性模量（254.4 GPa）。我们使用培养在涂层和未涂层​​样品上的兔骨髓基质细胞（BMSC）评估了TaN涂层的细胞相容性和成骨活性。膜的生物相容性及其对细胞增殖的影响通过活/死细胞染色和CCK-8分析进行了检查。扫描电镜和细胞骨架荧光染色观察不同样品的细胞黏附和形态。通过结节钙化染色和碱性磷酸酶定量分析评估了膜对成骨细胞活性的影响。我们证明TaN膜可以增强BMSCs的粘附，增殖和分化。这些结果表明，通过磁控溅射制备的TaN膜具有很大的潜力可应用于骨科。

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