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首页> 外文期刊>RSC Advances >A Ruthenium(II) complex as a potential luminescent switch-on probe for G-quadruplex DNA
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A Ruthenium(II) complex as a potential luminescent switch-on probe for G-quadruplex DNA

机译：钌（II）配合物作为G-四链体DNA的潜在发光开关探针

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This study aimed to synthesize two ruthenium(II) complexes modified with aryl alkynes [Ru(phen)2(TMSEPIP)](ClO4)2 (1, phen = 1,10-phenanthrolin; TMSEPIP = 2-(2-trimethylsilylethylphenyl)imidazole[4,5f][1,10] phenanthroline) and [Ru(phen)2(BEPIP)](ClO4)2 (2, BEPIP = 2-(2-phenethylphenyl)imidazole[4,5f][1,10]phenanthroline) and evaluate their ability to act as luminescent probes of c-myc G-quadruplex DNA. It was discovered that the luminescence of 1 decreased in the presence of c-myc G-quadruplex DNA, while the luminescence of 2 clearly increased under the same conditions. Besides, in the presence of double-stranded DNA, the luminescence intensity of 1 and 2 showed little obvious change. It was interesting to find by atomic force microscopy that 1 could promote c-myc G-quadruplex DNA self-assembly to form a nano-network structure, while 2 could promote the formation of a nanowire structure. Further studies showed that 1 was inserted into the groove formed by base pairs A6-G8 and G21-T23 of c-myc G-quadruplex DNA, while 2 was inserted into the groove formed by G7-G9 and G22-A24. It is speculated that 2 can be developed as a potential luminescent switch-on probe through selectively recognizing and promoting self-assembly of c-myc G-quadruplex DNA.
机译：这项研究旨在合成两个芳基炔烃[Ru（phen） 2 （TMSEPIP）]（ClO 4 ） 2 （1，phen = 1,10-菲咯啉; TMSEPIP = 2-（2-三甲基甲硅烷基乙基苯基）咪唑[4,5 f ] [1,10]菲咯啉）和[Ru（phen） 2 （BEPIP）]（ClO 4 ） 2 （2，BEPIP = 2-（2-苯乙基苯基）咪唑[4,5 f < / em>] [1,10]菲咯啉）并评估其作为 c-myc G-四链体DNA发光探针的能力。发现在存在 c-myc G-四链体DNA的情况下，1的发光减少，而在相同条件下，2的发光明显增加。此外，在双链DNA存在下，1和2的发光强度几乎没有明显变化。有趣的是，通过原子力显微镜发现1可以促进 c-myc G-四链体DNA自组装形成纳米网络结构，而2可以促进纳米线结构的形成。进一步的研究表明，将1个插入由 c-myc G-四链体DNA的碱基对A6-G8和G21-T23形成的凹槽中，将2插入由G7-G9和G22-A24。据推测，通过选择性识别和促进 c-myc G-四链体DNA的自组装，可以将2作为潜在的发光开关探针。

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