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首页> 外文期刊>RSC Advances >Co-production of microbial oil and exopolysaccharide by the oleaginous yeast Sporidiobolus pararoseus grown in fed-batch culture
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Co-production of microbial oil and exopolysaccharide by the oleaginous yeast Sporidiobolus pararoseus grown in fed-batch culture

机译：分批分批培养的产油酵母 Sporidiobolus pararoseus 共同生产微生物油和胞外多糖

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摘要

The production cost of microbial oil was reduced by improving the exopolysaccharide (EPS) production to share the production cost using Sporidiobolus pararoseus JD-2. Batch fermentation demonstrated that S. pararoseus JD-2 has the potential to co-produce oil and EPS with 120 g L?1 glucose, 20 g L?1 corn steep liquor and 10 g L?1 yeast extract as carbon and nitrogen sources. Using fed-batch fermentation for 72 h resulted in oil and EPS production of 41.6 ± 2.5 g L?1 and 13.1 ± 0.6 g L?1 with the productivity of 0.58 g L?1 h?1 and 0.182 g L?1 h?1, respectively. The fat soluble nutrients in the oil were studied, indicating that it was constituted of 79.19% unsaturated fatty acids and contained 505 mg per kg-oil of carotenoids. Moreover, the EPS contained only one type of polysaccharide; the main monosaccharide compositions were galactose, glucose and mannose in a proportion of 16?:?8?:?1. These results implied that EPS produced by S. pararoseus JD-2 was a new type of EPS.
机译：通过改善副糖 Sprosediobolus pararoseus JD-2来改善胞外多糖（EPS）的生产成本，从而降低了微生物油的生产成本。分批发酵表明 S。 JD-2可以与120 g L ？1 葡萄糖，20 g L ？葡萄糖联产石油和EPS。 1 玉米浆和10 g L ？1 酵母提取物作为碳和氮源。分批补料发酵72小时产生的油和EPS分别为41.6±2.5 g L ？1 和13.1±0.6 g L ？1 ，生产率为0.58 g L ？1 h ？1 和0.182 g g L ？1 h ？1 。对油中的脂溶性营养物质进行了研究，表明其由79.19％的不饱和脂肪酸组成，每千克油中的类胡萝卜素含量为505 mg。而且，EPS仅包含一种类型的多糖;其仅包含一种多糖。主要的单糖成分是半乳糖，葡萄糖和甘露糖，比例为16：：8：1。这些结果暗示 S产生的EPS。 pararoseus JD-2是一种新型EPS。

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