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首页> 外文期刊>RSC Advances >Co-production of microbial oil and exopolysaccharide by the oleaginous yeast Sporidiobolus pararoseus grown in fed-batch culture
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Co-production of microbial oil and exopolysaccharide by the oleaginous yeast Sporidiobolus pararoseus grown in fed-batch culture

机译：通过在FED分批培养中生长的烯胺酵母Sporidiobolus Pararoseus的微生物油和外偶联的共同生产

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摘要

The production cost of microbial oil was reduced by improving the exopolysaccharide (EPS) production to share the production cost using Sporidiobolus pararoseus JD-2. Batch fermentation demonstrated that S. pararoseus JD-2 has the potential to co-produce ad and EPS with 120 g L-1 gLucose, 20 g L-1 corn steep Liquor and 10 g L-1 yeast extract as carbon and nitrogen sources. Using fed-batch fermentation for 72 h resulted in oil and EPS production of 41.6 +/- 2.5 g L-1 and 13.1 +/- 0.6 g L-1 with the productivity of 0.58 g L-1 h(-1) and 0.182 g L-1 h(-1), respectively. The fat soluble nutrients in the oiL were studied, indicating that it was constituted of 79.19% unsaturated fatty acids and contained 505 mg per kg-oil of carotenoids. Moreover, the EPS contained only one type of polysaccharide; the main monosaccharide compositions were galactose, gLucose and mannose in a proportion of 16: 8: 1. These results implied that EPS produced by S. pararoseus JD-2 was a new type of EPS.
机译：通过改善外渗（EPS）生产来分享使用Sporidiobolus Pararoseus JD-2来减少微生物油的生产成本。 分批发酵证明了S. pararoseusJD-2具有共同产生的AD和EPS，120g L-1葡萄糖，20g L-1玉米溶液和10g L-1酵母提取物作为碳和氮源。 使用FED分批发酵72小时导致油和EPS产生41.6 +/- 2.5g L-1和13.1 +/- 0.6g L-1，其生产率为0.58g l-1 h（-1）和0.182 G L-1 H（-1）分别。 研究了油中的脂溶性营养物，表明它由79.19％的不饱和脂肪酸构成，并含有505mg的类胡萝卜素。 此外，EPS仅包含一种类型的多糖; 主要单糖组合物是半乳糖，葡萄糖和甘露糖，比例为16：8：1。这些结果暗示了由S. pararoseusJD-2产生的EPS是一种新型的EPS。

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  • 来源
    《RSC Advances》 |2018年第6期|共9页
  • 作者

    Han Mei; Xu Jian-Zhong; Liu Zhen-Min; Qian He; Zhang Wei-Guo;

  • 作者单位

    Jiangnan Univ Sch Biotechnol Key Lab Carbohydrate Chem &

    Biotechnol Minist Educ 1800 Lihu Rd Wuxi 214122 Jiangsu Peoples R China;

    Jiangnan Univ Sch Biotechnol Key Lab Carbohydrate Chem &

    Biotechnol Minist Educ 1800 Lihu Rd Wuxi 214122 Jiangsu Peoples R China;

    Technol Ctr Bright Dairy &

    Food Co Ltd State Key Lab Dairy Biotechnol 1518 Jiangchang West Rd Shanghai 200436 Peoples R China;

    Jiangnan Univ Sch Food Sci &

    Technol 1800 Lihu Rd Wuxi 214122 Jiangsu Peoples R China;

    Jiangnan Univ Sch Biotechnol Key Lab Carbohydrate Chem &

    Biotechnol Minist Educ 1800 Lihu Rd Wuxi 214122 Jiangsu Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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