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首页> 外文期刊>RSC Advances >Preparation of curcuminoid microemulsions from Curcuma longa L. to enhance inhibition effects on growth of colon cancer cells HT-29
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Preparation of curcuminoid microemulsions from Curcuma longa L. to enhance inhibition effects on growth of colon cancer cells HT-29

机译：姜黄中姜黄素微乳的制备以增强对结肠癌细胞HT-29生长的抑制作用

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The objectives of this study were to extract curcuminoid from Curcuma longa L. (C. longa), a vital medicinal plant demonstrated to possess many biological activities, and prepare the curcuminoid extract and microemulsion for studying the inhibition mechanism of HT-29 colon cancer cells. Results showed that a total of 3 curcuminoids including curcumin, demethoxycurcumin (DMC) and bisdemethoxycurcumin (BDMC), were separated within 10 min by using an Eclipse XDB-18 column and a gradient mobile phase of 0.1% formic acid solution (A) and acetonitrile (B). The curcuminoid microemulsion composed of soybean oil, Tween 80, ethanol and water was prepared with a high stability and mean particle size of 10.9 nm, zeta-potential of ?65.2 mV and encapsulation efficiency of 85.7%. Both curcuminoid extract and microemulsion were effective in inhibiting HT-29 cell growth with the IC50 being 3.83 and 2.51 μg mL?1 after 24 h incubation, respectively, but further reduced to 2.23 and 1.94 μg mL?1, after 48 h incubation. Both treatments could decrease the proportion of both viable and necrosis cells and increase the proportion of both early and late apoptosis cells in a dose-dependent manner, with the cell cycle arrested at the S phase. Also, both treatments could up-regulate p53 expression and down-regulate cyclin A and CDK2 expressions through a p21-independent pathway. In addition, the expressions of Bax and cytochrome C as well as the activities of caspase-8, caspase-9 and caspase-3 increased for the curcuminoid extract, while the curcuminoid microemulsion showed the same trend with the exception that an insignificant change (p > 0.05) in Bax expression was observed. Collectively, this study demonstrated that the curcuminoid microemulsion prepared from C. longa may possess great potential for the treatment of colon cancer in the future.
机译：这项研究的目的是从被证明具有许多生物学活性的重要药用植物 Curcuma longa L.（ C。longa ）中提取姜黄素，并制备姜黄素提取物和微乳液用于研究HT-29结肠癌细胞的抑制机制。结果显示，使用Eclipse XDB-18色谱柱和0.1％甲酸溶液（A）和乙腈的梯度流动相，在10分钟内分离出了3种姜黄素，包括姜黄素，去甲氧基姜黄素（DMC）和双去甲氧基姜黄素（BDMC）。 （B）。制备了由大豆油，吐温80，乙醇和水组成的类姜黄素微乳，具有高稳定性，平均粒径为10.9 nm，ζ电位为〜65.2 mV，包封效率为85.7％。姜黄素提取物和微乳剂均能有效抑制HT-29细胞生长，IC 50 为3.83和2.51μgmL ？1 孵育24小时后，但孵育48小时后进一步降低至2.23和1.94μgmL ？1 。两种处理均可以剂量依赖的方式降低存活细胞和坏死细胞的比例，并增加早期和晚期凋亡细胞的比例，并且细胞周期停滞在S期。而且，两种治疗都可以通过非p21依赖性途径上调p53表达，并下调cyclin A和CDK2表达。此外，姜黄素提取物的Bax和细胞色素C的表达以及caspase-8，caspase-9和caspase-3的活性增加，而姜黄素微乳液显示出相同的趋势，只是变化不明显（< em> p C制备的。 longa 在将来可能具有治疗结肠癌的巨大潜力。

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