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首页> 外文期刊>RSC Advances >Activity and stability analysis of covalent conjugated lysozyme-single walled carbon nanotubes: potential biomedical and industrial applications
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Activity and stability analysis of covalent conjugated lysozyme-single walled carbon nanotubes: potential biomedical and industrial applications

机译：共价共轭溶菌酶单壁碳纳米管的活性和稳定性分析：潜在的生物医学和工业应用

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Conjugated protein–carbon nanotubes possess unique physicochemical properties that make them attractive to a wide range of applications. We explored the effects of covalent conjugation of lysozymes with single-walled carbon nanotubes (SWCNTs) on its activity and stability. Lysozyme was conjugated onto carboxyl-functionalized SWCNTs through carbodiimide method. Post conjugation changes of the enzyme were investigated using fluorescence measurements to generate plots of protein unfolding at 0 and 6 M guanidine hydrochloride (Gn-HCl) concentrations. Free lysozyme showed a remarkable increase in the fluorescence intensity at 287 nm in addition to a red shift from 343 to 352 nm. The emission spectrum of conjugated lysozyme showed a significant increase in the fluorescence intensity at 287 nm and a significant decrease in intensity at 348 nm. These results confirmed that both tryptophan and phenylalanine residues have an important role in the fluorescence bulk of both free and conjugated lysozymes. Kinetic parameters of free and conjugated lysozyme activities (KM and Vmax), optimum pH, thermal and pH stability, and stability in the presence of denaturant agents such as urea and salts were investigated. Changes were observed in KM and Vmax from 4.8 to 5.6 mM and 193 to 197 nmol min?1, respectively. Conjugated lysozyme showed a remarkable increase in pH stability in a range of 3.0 to 10.0 at 70 °C. The results showed first-order inactivation kinetics for both forms of lysozyme with a rate constant of about 0.139 min?1 for 10 minutes of incubation at 70 °C in the presence of KCl and KSCN.
机译：共轭蛋白碳纳米管具有独特的理化特性，使其对广泛的应用具有吸引力。我们探索了溶菌酶与单壁碳纳米管（SWCNTs）的共价结合对其活性和稳定性的影响。通过碳二亚胺法将溶菌酶偶联到羧基官能化的SWCNT上。使用荧光测量研究了酶的共轭后变化，以生成在0和6 M盐酸胍（Gn-HCl）浓度下蛋白质解折叠的图。游离溶菌酶显示出从343 nm到352 nm的红移以及287 nm处荧光强度的显着增加。共轭溶菌酶的发射光谱显示在287 nm处的荧光强度显着增加，在348 nm处的强度显着下降。这些结果证实，色氨酸和苯丙氨酸残基在游离和结合的溶菌酶的荧光中都具有重要作用。游离和共轭溶菌酶活性的动力学参数（ K M 和 V max ），最佳pH，热和pH稳定性以及在变性剂（例如尿素和盐）存在下的稳定性。在 K M 和 V max 分别为4.8至5.6 mM和193至197 nmol min ？1 。共轭溶菌酶在70°C下的pH稳定性在3.0至10.0的范围内显着增加。结果表明，两种形式的溶菌酶的一级失活动力学均以约0.139 min ？1 的速率常数进行，在70°C的温度下于20°C孵育10分钟。 KCl和KSCN。

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