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Effects of nano-grooved gelatin films on neural induction of human adipose-derived stem cells

机译：纳米沟槽明胶膜对人脂肪干细胞神经诱导的影响

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摘要

The extra cellular matrix (ECM) and cell–cell interactions facilitate the survival, self-renewing and differentiation capabilities of stem cells. Biomaterials with specific structures such as grooves, ridges, pits, or pillars can mimic the topographic landscape of the niche. Cells can “sense” the mechanical properties and surface patterns, ranging from the micro- to nano-scale, of the substrate; hence, different sizes of nano-grooves on a gelatin surface were designed. The design of grooves can be systematically modified and those structures can reflect the organization of the ECM. In previous studies, polystyrene (PS) was often used because it is easy to fabricate topographic structures. For better biocompatibility, gelatin was chosen and fabricated into ideal nano-groove films. On the other hand, gelatin can be crosslinked by using several crosslinking agents, which leads to a higher mechanical strength and better flexibility. It was known that stem cells can serve as a source of neurons in transplantation therapies. The differentiation of neurons is associated with directionality of stem cell. To investigate the effect of topographic cues on stem cells, groove pattern arrays were constructed onto gelatin surfaces. Human adipose-derived stem cells (hASCs) were seeded onto the patterned gelatin films to observe cell proliferation and differentiation.
机译：细胞外基质（ECM）和细胞间的相互作用促进了干细胞的存活，自我更新和分化能力。具有特定结构（例如凹槽，山脊，凹坑或柱子）的生物材料可以模仿利基的地形景观。细胞可以“感知”基材的机械性能和表面图案，范围从微米到纳米。因此，在明胶表面设计了不同尺寸的纳米沟槽。凹槽的设计可以被系统地修改，并且那些结构可以反映ECM的组织。在以前的研究中，经常使用聚苯乙烯（PS），因为它易于制造形貌结构。为了获得更好的生物相容性，选择了明胶并将其制成理想的纳米凹槽膜。另一方面，可以通过使用几种交联剂使明胶交联，这导致更高的机械强度和更好的柔韧性。众所周知，干细胞可以在移植疗法中充当神经元的来源。神经元的分化与干细胞的方向性有关。为了研究地形线索对干细胞的影响，在明胶表面上构建了凹槽图案阵列。将人脂肪来源的干细胞（hASC）播种到带图案的明胶膜上，以观察细胞的增殖和分化。

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