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首页> 外文期刊>RSC Advances >Poly(L-lysine) modified zein nanofibrous membranes as efficient scaffold for adhesion, proliferation, and differentiation of neural stem cells
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Poly(L-lysine) modified zein nanofibrous membranes as efficient scaffold for adhesion, proliferation, and differentiation of neural stem cells

机译：聚（L-赖氨酸）修饰的玉米醇溶蛋白纳米纤维膜作为神经干细胞粘附，增殖和分化的有效支架

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Excellent biocompatibility and bioactivity are necessary requirements for a scaffold for nerve repair and regeneration. Natural plant protein zein was chosen as the primary material and poly(L-lysine), which is composed of common amino acids in the human body, was used to modify it. Poly(L-lysine) modified zein (ZPLL) with different PLL contents of 1.46%, 3.57%, and 6.18% was synthesized and nanofibrous membranes were prepared by electrospinning. The hydrophilicity of the membranes improved with an increase of PLL content. The biodegradability of the membranes was proved by in vitro experiments. Compared with pure zein membranes, ZPLL membranes can efficiently improve cell viability, adhesion, proliferation, and differentiation of neural stem cells (NSCs) and the effect of PLL content was further investigated. The results show that when the PLL content was 3.57%, cell adhesion and proliferation proved to be the best and most differentiated toward mature neurons with extensive neurite formation and astrocytes rather than oligodendrocytes. The ZPLL was made into nerve conduits for future study and they may be a promising biomaterial for nerve repair and regeneration.
机译：出色的生物相容性和生物活性是神经修复和再生支架的必要条件。以天然植物蛋白玉米醇溶蛋白为主要原料，并使用由人体中常见氨基酸组成的聚 L -赖氨酸进行修饰。合成了不同PLL含量分别为1.46％，3.57％和6.18％的聚（L> L -赖氨酸）玉米醇溶蛋白（ZPLL），并通过静电纺丝制备了纳米纤维膜。膜的亲水性随PLL含量的增加而提高。通过体外实验证明了膜的生物降解性。与纯玉米蛋白膜相比，ZPLL膜可有效提高神经干细胞（NSCs）的细胞活力，粘附，增殖和分化，并进一步研究了PLL含量的影响。结果表明，当PLL含量为3.57％时，细胞粘附和增殖被证明是最好的，并且最分化为具有广泛的神经突形成和星形胶质细胞而不是少突胶质细胞的成熟神经元。 ZPLL被制成神经导管以备将来研究，它们可能是用于神经修复和再生的有前途的生物材料。

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