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Two-stage mass spectrometry approach for the analysis of triterpenoid glycosides in Fagonia indica

机译：两级质谱法分析F米中的三萜苷

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Triterpenoid glycosides are molecules widely distributed in plants and have shown a wide range of biological activities against various diseases. This paper describes the qualitative and quantitative analysis of triterpenoid glycoside (saponins) using a two-stage mass spectrometry approach in five samples of Fagonia indica collected from various parts of the country. In the first stage, triterpenoid glycosides were identified using liquid chromatography high-resolution mass spectrometry using UHPLC-QTOF-MS system. In the second stage, compounds were quantified using a multiple reaction monitoring (MRM) approach using an UHPLC-QQQ-MS system. Fagonia indica has shown a wide range of biological activities and found to be rich in saponin or triterpenoid glycoside constituents. A total of thirteen triterpenoid saponins were identified based on high-resolution analysis, MS/MS and database comparison, while six of them were simultaneously quantified using the multiple reaction monitoring (MRM) approach. The results indicate that the samples share a similar UHPLC pattern, however, the amount of these saponins in samples varies greatly. Compound 4 i.e. nayabin D was the major constituent (1.4–3.8 μg g ~(?1) ) among the six analyzed compounds. The results demonstrated that the developed multi-compound determination in combination with a fingerprint analysis method is rapid, accurate, precise and sensitive and can be utilized for quality control and high-throughput quantification of various saponins in Fagonia indica may be extended to other plant species.
机译：三萜类糖苷是广泛分布在植物中的分子，并已显示出对各种疾病的广泛生物活性。本文描述了使用两阶段质谱法对从该国各地收集的五种of属植物样品中三萜类苷（皂苷）的定性和定量分析。在第一阶段，使用UHPLC-QTOF-MS系统通过液相色谱高分辨率质谱法鉴定三萜类糖苷。在第二阶段，使用UHPLC-QQQ-MS系统使用多反应监测（MRM）方法对化合物进行定量。 agon木已显示出广泛的生物活性，并发现其富含皂苷或三萜糖苷成分。根据高分辨率分析，MS / MS和数据库比较，共鉴定出13种三萜皂苷，同时使用多反应监测（MRM）方法同时定量了其中的6种。结果表明样品具有相似的UHPLC模式，但是样品中这些皂苷的含量差异很大。在六个被分析的化合物中，化合物4即nayabin D是主要成分（1.4–3.8μgg〜（？1））。结果表明，结合指纹图谱分析方法开发的多种化合物测定方法快速，准确，准确，灵敏，可用于质控，并且对F木中各种皂苷的高通量定量分析可扩展到其他植物。 。

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