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Conversion of corn stover alkaline pre-treatment waste streams into biodiesel via Rhodococci

机译:玉米秸秆碱预处理废物流通过红球菌转化为生物柴油

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The bioconversion of second-generation cellulosic ethanol waste streams into biodiesel via oleaginous bacteria is a novel optimization strategy for biorefineries with substantial potential for rapid development. In this study, one- and two-stage alkali/alkali-peroxide pretreatment waste streams of corn stover were separately implemented as feedstocks in 96 h batch reactor fermentations with wild-type Rhodococcus opacus PD 630, R. opacus DSM 1069, and R. jostii DSM 44719T. Here we show using 31P-NMR, HPAEC-PAD, and SEC analyses, that the more rigorous and chemically-efficient two-stage chemical pretreatment effluent provided higher concentrations of solubilized glucose and lower molecular weight (~70–300 g mol?1) lignin degradation products thereby enabling improved cellular density, viability, and oleaginicity in each respective strain. The most significant yields were by R. opacus PD 630, which converted 6.2% of organic content with a maximal total lipid production of 1.3 g L?1 and accumulated 42.1% in oils based on cell dry weight after 48 h.
机译:通过产油细菌将第二代纤维素乙醇废物流生物转化为生物柴油是生物提炼厂的一种新型优化策略,具有快速发展的巨大潜力。在这项研究中,玉米秸秆的一阶段和两阶段碱/碱金属过氧化物预处理废物流分别作为野生型 Rhodococcus opacus PD 630, R。 opacus DSM 1069和 R。 jostii DSM 44719 T 。在这里,我们显示使用 31 P-NMR,HPAEC-PAD和SEC分析,化学处理效率更高,化学效率更高的两阶段化学预处理流出物提供了更高的溶解度葡萄糖和较低的分子量(〜70–300 g mol ?1 )木质素降解产物,从而能够提高每种菌株的细胞密度,生存力和油脂性。最显着的产量由 R决定。 opacus PD 630,可转化6.2%的有机物,最大总脂质产量为1.3 g L ?1 ,在基于细胞的油中累积42.1% 48小时后干重。

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