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首页> 外文期刊>RSC Advances >PHBV/bioglass composite scaffolds with co-cultures of endothelial cells and bone marrow stromal cells improve vascularization and osteogenesis for bone tissue engineering
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PHBV/bioglass composite scaffolds with co-cultures of endothelial cells and bone marrow stromal cells improve vascularization and osteogenesis for bone tissue engineering

机译：PHBV /生物玻璃复合支架与内皮细胞和骨髓基质细胞共培养可改善血管生成和骨生成，用于骨组织工程

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Polyhydroxybutyrate–polyhydroxyvalerate (PHBV) and bioglass (BG) have been widely reported to be suitable for bone tissue engineering. However, composite scaffolds with polymers and bioceramics have shown advantages over pure polymer and bioceramic scaffolds for bone tissue engineering. In addition, recent studies have shown that cross-talk between endothelial cells and osteoblastic cells can stimulate bone regeneration compared to tissue engineering constructs containing only one type of cell. Therefore, in this study, we aim to construct an improved engineered bone containing PHBV/BG composite scaffold with co-cultures of human umbilical vein endothelial cells (HUVECs) and human bone marrow stromal cells (HBMSCs) in order to enhance osteogenesis and angiogenesis of bone repair. Results showed that addition of BG into PHBV could enhance osteogenic differentiation of co-cultured HBMSCs and vascularization of co-cultured HUVECs by upregulating paracrine effects between the two types of cells compared to pure PHBV scaffolds. Among all groups, composite scaffolds containing PHBV with 10% BG showed the strongest stimulatory effects on osteogenic differentiation and vascularization due to their appropriate ion products, specifically, the appropriate concentration of silicon ions. In vivo results also demonstrated that PHBV containing 10% BG scaffolds with co-cultures of HUVECs and HBMSCs showed the strongest stimulatory effects on osteogenesis and angiogenesis among all groups. Taken together, PHBV/BG scaffolds with co-cultures of endothelial cells and osteogenic cells possess great application potential for bone tissue engineering.
机译：聚羟基丁酸酯–聚羟基戊酸酯（PHBV）和生物玻璃（BG）被广泛报道适用于骨组织工程。然而，用于骨组织工程的具有聚合物和生物陶瓷的复合支架已经显示出优于纯聚合物和生物陶瓷支架的优势。此外，最近的研究表明，与仅包含一种类型细胞的组织工程构建体相比，内皮细胞与成骨细胞之间的串扰可以刺激骨骼再生。因此，在这项研究中，我们旨在构建改良的含有PHBV / BG复合支架的工程骨，并与人脐静脉内皮细胞（HUVEC）和人骨髓基质细胞（HBMSC）共同培养，以增强其成骨和血管生成骨修复。结果表明，与纯PHBV支架相比，在PHBV中添加BG可以通过上调两种细胞之间的旁分泌效应来增强共培养的HBMSC的成骨分化和共培养的HUVEC的血管形成。在所有组中，含PHBV和BG 10％的复合支架由于其适当的离子产物，特别是适当的硅离子浓度，对成骨分化和血管生成具有最强的刺激作用。 体内结果还表明，在HUVEC和HBMSC的共培养物中，含10％BG支架的PHBV对所有组的成骨和血管生成均具有最强的刺激作用。总之，PHBV / BG支架与内皮细胞和成骨细胞的共培养物在骨组织工程中具有巨大的应用潜力。

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