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首页> 外文期刊>RSC Advances >Alginate oligosaccharide enhances intestinal integrity of weaned pigs through altering intestinal inflammatory responses and antioxidant status
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Alginate oligosaccharide enhances intestinal integrity of weaned pigs through altering intestinal inflammatory responses and antioxidant status

机译：海藻酸钠低聚糖通过改变肠道炎症反应和抗氧化状态来增强断奶猪的肠道完整性

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Alginate oligosaccharide (AOS), prepared from depolymerised alginate, a natural polysaccharide occurring in the cell walls of brown algae, provides beneficial effects for intestinal health. However, the underlying mechanisms by which AOS supplementation maintains the intestinal integrity of weaned pigs remain obscure. Here, we aimed to determine how AOS modulates the intestinal integrity of weaned pigs. Twenty-four weaned pigs were assigned to two treatments: a control group (basal diet) and an AOS group (the basal diet supplemented with 100 mg kg ~(?1) AOS). On day 15, eight pigs per treatment were randomly selected and sacrificed for serum and intestinal samples. We observed that AOS supplementation enhanced the intestinal integrity, as evidenced by the increased ( P < 0.05) intestinal occludin protein abundance. Compared to the control group, AOS ingestion both elevated ( P < 0.05) the jejunal and ileal catalase activity and decreased ( P < 0.05) the duodenal and jejunal tumour necrosis factor-α concentration and mast cell tryptase expression. Furthermore, AOS down-regulated ( P < 0.05) the duodenal toll-like receptor 4 (TLR4) and its down-stream signals, myeloid differentiation factor 88 (MyD88), interleukin-1 receptor-associated kinase 1 (IRAK1) and tumour necrosis factor receptor-associated factor 6 (TRAF6) mRNA levels, as well as jejunal nucleotide-binding oligomerisation domain protein 1 (NOD1) and its adaptor molecule, receptor-interacting serine/threonine-protein kinase 2 (RIPK2), mRNA levels. Additionally, phospho-nuclear factor-κB (p-NF-κB) p65 protein abundance in the duodenum and jejunum was down-regulated ( P < 0.05) following AOS supplementation. According to the above results, the enhanced intestinal integrity in AOS-supplemented pigs appears to be associated with the elevated antioxidant capacity and the reduced mast cell degranulation, as well as the inhibited pro-inflammatory cytokines production via inhibiting the TLR4/NF-κB and NOD1/NF-κB signalling pathways.
机译：由解聚的藻酸盐（一种存在于褐藻细胞壁中的天然多糖）制得的藻酸盐低聚糖（AOS）为肠道健康提供了有益的作用。然而，补充AOS维持断奶猪肠道完整性的基本机制仍然不清楚。在这里，我们旨在确定AOS如何调节断奶猪的肠道完整性。 24只断奶猪接受两种处理：对照组（基础日粮）和AOS组（基础日粮补充100 mg kg〜（？1）AOS）。在第15天，每次处理随机选择八只猪，并处死其血清和肠样品。我们观察到，AOS补充剂增强了肠的完整性，这由增加的肠阻塞蛋白（P <0.05）所证明。与对照组相比，摄入AOS可使空肠和回肠过氧化氢酶活性升高（P <0.05），而十二指肠和空肠肿瘤坏死因子-α浓度和肥大细胞类胰蛋白酶的表达均降低（P <0.05）。此外，AOS下调了十二指肠toll样受体4（TLR4）及其下游信号，髓样分化因子88（MyD88），白介素1受体相关激酶1（IRAK1）和肿瘤坏死（P <0.05）。因子受体相关因子6（TRAF6）mRNA水平，空肠核苷酸结合寡聚域蛋白1（NOD1）及其衔接分子，受体相互作用丝氨酸/苏氨酸蛋白激酶2（RIPK2）mRNA水平。此外，补充AOS后十二指肠和空肠中的磷酸核因子-κB（p-NF-κB）p65蛋白丰度被下调（P <0.05）。根据以上结果，补充AOS的猪的肠道完整性增强似乎与抗氧化能力增强和肥大细胞脱粒减少有关，并通过抑制TLR4 /NF-κB和SPAR抑制了促炎性细胞因子的产生。 NOD1 /NF-κB信号通路。

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