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首页> 外文期刊>RSC Advances >Blocking celiac antigenicity of the glutamine-rich gliadin 33-mer peptide by microbial transglutaminase
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Blocking celiac antigenicity of the glutamine-rich gliadin 33-mer peptide by microbial transglutaminase

机译：通过微生物转谷氨酰胺酶阻断富含谷氨酰胺的麦醇溶蛋白33聚体肽的腹腔抗原性

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Celiac disease (CD) is a T cell-mediated enteropathy of the small intestine and caused by the ingestion of wheat gluten and related prolamins in barley and rye. However, there is no effective therapy to alleviate symptoms of celiac disease except for a life-long gluten-free diet. Recent studies showed that modification by microbial transglutaminase (mTG) could reduce the gliadin-specific immune response. In the present study, different acyl-acceptor substrates in combination with mTG were used to modify the model 33-mer peptide (LQLQPFPQPQLPYPQPQLPYPQPQLPYPQPQPF), which is a particular celiac toxic α-gliadin peptide. RP-HPLC and LC-ESI-MS were performed to test the extent of the modifications. R5 ELISA and G12 ELISA were used to analyze the antigenicity of the modified peptide. The shifts of retention time and molecular weight showed great modification of 33-mer peptide after 2 h of incubation with mTG. When acyl-acceptor substrates were crosslinked with 33-mer peptide, the antigenicity of modified peptide forms was decreased compared to its initial level. In summary, it is demonstrated that mTG is active on a variety of chemically acyl-acceptor substrates. Transamidation by mTG with an appropriate amine donor can be used to block the antigenicity of gliadin peptide related to celiac disease. These findings highlight a potential strategy to prevent cereal toxicity in celiac disease.
机译：腹腔疾病（CD）是T细胞介导的小肠肠病，由大麦和黑麦中的小麦面筋和相关谷醇溶蛋白的摄入引起。但是，除了终生无麸质饮食外，没有任何有效的疗法可减轻腹腔疾病的症状。最近的研究表明，微生物转谷氨酰胺酶（mTG）的修饰可以减少麦醇溶蛋白特异性的免疫反应。在本研究中，将不同的酰基受体底物与mTG结合使用来修饰33-mer模型肽（LQLQPFPQPQLPYPQPQLPYPQPQLPYPQPQPF），这是一种特殊的腹腔毒性α-麦醇溶蛋白肽。进行了RP-HPLC和LC-ESI-MS以测试修饰的程度。 R5 ELISA和G12 ELISA用于分析修饰肽的抗原性。与mTG孵育2小时后，保留时间和分子量的变化显示33-mer肽的巨大修饰。当酰基受体底物与33-mer肽交联时，与其初始水平相比，修饰的肽形式的抗原性降低。总而言之，证明了mTG在多种化学酰基受体底物上具有活性。 mTG与适当的胺供体的氨基转移可用于阻断与乳糜泻有关的麦醇溶蛋白肽的抗原性。这些发现强调了预防谷类疾病对乳糜泻的潜在策略。

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