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首页> 外文期刊>RSC Advances >Production of cellulase by Trichoderma reesei from pretreated straw and furfural residues
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Production of cellulase by Trichoderma reesei from pretreated straw and furfural residues

机译：里氏木霉从预处理的稻草和糠醛残留物中生产纤维素酶

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In this study, furfural residues were used as a substrate for cellulase production by the fungi Trichoderma reesei . The results indicated that a low pH and the presence of lignin in the furfural residues have an obvious impact on cellulase production by T. reesei . After pH adjustment, furfural residues could be used for cellulase production by T. reesei , with a higher filter paper activity (FPA) and a higher activity of CMCase compared to that yielded from furfural residues with pH unadjusted. After being washed with 1.6% (w/v) H _(2) O _(2) , all of the lignin in the furfural residues was removed, and an FPA of 7.1 FPU ml ~(?1) and a CMCase activity of 3.4 IU ml ~(?1) were obtained in 115 h, while pretreated straw could yield an FPA of 8.0 FPU ml ~(?1) and a CMCase activity of 2.7 IU ml ~(?1) in 160 h. Moreover, after being treated with H _(2) O _(2) , furfural residues could be used as an inducer in the production of cellulases. With the treated furfural residues added into the medium at the beginning of cultivation, T. reesei gave the maximum FPA (8.4 FPU ml ~(?1) ) and CMCase activity (4.8 IU ml ~(?1) ) at 142 h from pretreated straw, which is relatively high for cellulase production compared to that from most other agricultural wastes reported.
机译：在这项研究中，糠醛残留物被用作里氏木霉属真菌产生纤维素酶的底物。结果表明，低pH值和糠醛残留物中木质素的存在对里氏木霉纤维素酶的生产有明显的影响。调节pH后，糠醛残留物可用于里氏木霉生产纤维素酶，与未经调节pH的糠醛残留物相比，具有更高的滤纸活性（FPA）和更高的CMCase活性。用1.6％（w / v）H _（2）O _（2）洗涤后，糠醛残留物中的所有木质素均被去除，FPA为7.1 FPU ml〜（？1），CMCase活性为在115小时内获得3.4 IU ml〜（？1），而预处理的秸秆在160小时内可产生8.0 FPU ml〜（？1）的FPA和2.7 IU ml〜（？1）的CMCase活性。此外，在用H _（2）O _（2）处理后，糠醛残留物可以用作纤维素酶生产中的诱导剂。在培养开始时将处理过的糠醛残留物添加到培养基中，里氏木霉在预处理后142 h时获得最大FPA（8.4 FPU ml〜（？1））和CMCase活性（4.8 IU ml〜（？1））。与报道的大多数其他农业废料相比，秸秆的纤维素酶生产量相对较高。

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