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首页> 外文期刊>RSC Advances >Production of cellulase by Trichoderma reesei from pretreated straw and furfural residues
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Production of cellulase by Trichoderma reesei from pretreated straw and furfural residues

机译：从预处理稻草和糠醛残留的Trichoderma Reesei生产纤维素酶

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摘要

In this study, furfural residues were used as a substrate for cellulase production by the fungi Trichoderma reesei. The results indicated that a low pH and the presence of lignin in the furfural residues have an obvious impact on cellulase production by T. reesei. After pH adjustment, furfural residues could be used for cellulase production by T. reesei, with a higher filter paper activity (FPA) and a higher activity of CMCase compared to that yielded from furfural residues with pH unadjusted. After being washed with 1.6% (w/v) H2O2, all of the lignin in the furfural residues was removed, and an FPA of 7.1 FPU ml(-1) and a CMCase activity of 3.4 IU ml(-1) were obtained in 115 h, while pretreated straw could yield an FPA of 8.0 FPU ml(-1) and a CMCase activity of 2.7 IU ml(-1) in 160 h. Moreover, after being treated with H2O2, furfural residues could be used as an inducer in the production of cellulases. With the treated furfural residues added into the medium at the beginning of cultivation, T. reesei gave the maximum FPA (8.4 FPU ml(-1)) and CMCase activity (4.8 IU ml(-1)) at 142 h from pretreated straw, which is relatively high for cellulase production compared to that from most other agricultural wastes reported.
机译：在这项研究中，糠醛残基被用作由真菌里氏木霉纤维素酶生产的衬底。结果表明，低pH和木质素中的糠醛的残基中存在有由里氏木霉纤维素酶的生产有明显的影响。 pH调节后，可以使用糠醛残基的纤维素酶生产由里氏木霉，具有较高的滤纸活性（FPA）和相比于从与未调pH糠醛残基，得到羧甲基纤维素酶活的更高的活性。用1.6％洗涤后（W / V）H 2 O 2，在所有的糠醛残基木质素的除去，并在得到的7.1 FPU ml的FPA（-1）和3.4 IU ml的CMCase活性（-1） 115小时，而预处理的秸杆可产生8.0毫升FPU（-1）的FPA和2.7 IU毫升（-1）在160 h的CMC酶活性。此外，与正在治疗的H 2 O 2后，糠醛渣可以被用作在生产纤维素酶的诱导物。与处理过糠醛残基加入到在培养开始时培养基中，里氏木霉，得到最大FPA（8.4毫升FPU（-1））和CMCase活性（IU 4.8毫升（-1））以从经预处理的秸杆142小时，这是比较高的纤维素酶生产相比大多数其他农业废弃物的报道。

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  • 来源
    《RSC Advances》 |2018年第63期|共6页
  • 作者

    Zhao Chun Hai; Liu Xiaoyan; Zhan Tong; He Jianlong;

  • 作者单位

    Binzhou Polytech Binzhou 256603 Shandong Peoples R China;

    Huaiyin Normal Univ Jiangsu Key Lab Biomass Based Energy &

    Enzyme Tec Huaian 223300 Peoples R China;

    Huaiyin Normal Univ Jiangsu Key Lab Biomass Based Energy &

    Enzyme Tec Huaian 223300 Peoples R China;

    Baimai Green Bioenergy Co LTD Huaian 223300 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
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