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首页> 外文期刊>RSC Advances >Endophytic fungus Pseudofusicoccum stromaticum produces cyclopeptides and plant-related bioactive rotenoids
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Endophytic fungus Pseudofusicoccum stromaticum produces cyclopeptides and plant-related bioactive rotenoids

机译：内生真菌基质假单胞菌产生环肽和与植物有关的生物活性类胡萝卜素

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In the present study, we integrated liquid chromatography high-resolution mass spectrometry (LC-HRMS) and high-throughput DNA sequencing for prospecting cytotoxic specialized metabolites from Pseudofusicoccum stromaticum , an endophytic fungus associated to the medicinal plant Myracrodruon urundeuva . LC-HRMS profiling allowed identifying putatively eleven compounds in the ethyl acetate extract from P. stromaticum broth. Additionally, a chemical fractionation guided by cytotoxicity combined with spectrometric analysis resulted in the isolation of three compounds: the cyclopeptide cyclo- L -Phe- D -Leu- L -Leu- L -Leu- L -lle along with the known rotenoids rotenolone and tephrosin. MTT assay showed that tephrosin (IC _(50) 0.51 μg mL ~(?1) ) has strong cytotoxic effect and may be pointed out as the compound responsible for the antiproliferative activity of P. stromaticum . Next Generation Sequencing (NGS) and genome mining of P. stromaticum draft genome revealed 56 contigs codifying specialized metabolites biosynthesis-related enzymes. Nearly half of such genes (44.6%) could be mapped to orphan Biosynthetic Gene Clusters (BGCs) of related plant pathogens belonging to family Botryosphaeriaceae. Also, screening for rotenoids biosynthetic enzymes led to characterization of a putative chalcone isomerase-like (CHI-like) protein. This is the first report of rotenoids biosynthesized by a fungus, unveiling a unique ability of P. stromaticum .
机译：在本研究中，我们整合了液相色谱高分辨率质谱（LC-HRMS）和高通量DNA测序技术，用于寻找基质假单胞菌（一种与药用植物Myracrodruon urundeuva相关的内生真菌）的细胞毒性专门代谢产物。 LC-HRMS谱图分析可鉴定出间质毕生肉汤乙酸乙酯提取物中的11种化合物。另外，以细胞毒性和光谱分析为指导的化学分级分离分离出了三种化合物：环肽cyclo-L -Phe- D -Leu-L -Leu-L -Leu-L -lle以及已知的类胡萝卜素鱼藤酮和球蛋白。 MTT分析表明，去氧肾上腺素（IC _（50）0.51μgmL〜（？1））具有很强的细胞毒性作用，可能被指出是负责基质腐菌抗增殖活性的化合物。间质疟原虫草案基因组的下一代测序（NGS）和基因组挖掘揭示了56个重叠群，它们编码特殊的代谢产物生物合成相关酶。这些基因中将近一半（44.6％）可以定位到属于球形葡萄球菌科的相关植物病原体的孤儿生物合成基因簇（BGC）。同样，对类胡萝卜素生物合成酶的筛选导致了推定的查尔酮异构酶样（CHI样）蛋白的表征。这是首次由真菌生物合成类胡萝卜素的报道，揭示了间质疟原虫的独特能力。

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