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首页> 外文期刊>RSC Advances >Endophytic fungus Pseudofusicoccum stromaticum produces cyclopeptides and plantrelated bioactive rotenoids
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Endophytic fungus Pseudofusicoccum stromaticum produces cyclopeptides and plantrelated bioactive rotenoids

机译：内生真菌假血清族硅藻土产生环肽和血管相关的生物活性转位

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In the present study, we integrated liquid chromatography high-resolution mass spectrometry (LC-HRMS) and high-throughput DNA sequencing for prospecting cytotoxic specialized metabolites from Pseudofusicoccum stromaticum, an endophytic fungus associated to the medicinal plant Myracrodruon urundeuva. LC-HRMS profiling allowed identifying putatively eleven compounds in the ethyl acetate extract from P. stromaticum broth. Additionally, a chemical fractionation guided by cytotoxicity combined with spectrometric analysis resulted in the isolation of three compounds: the cyclopeptide cyclo-l-Phe-d-Leu-l-Leu-l-Leu-l-lle along with the known rotenoids rotenolone and tephrosin. MTT assay showed that tephrosin (IC50 0.51 g mL(-1)) has strong cytotoxic effect and may be pointed out as the compound responsible for the antiproliferative activity of P. stromaticum. Next Generation Sequencing (NGS) and genome mining of P. stromaticum draft genome revealed 56 contigs codifying specialized metabolites biosynthesis-related enzymes. Nearly half of such genes (44.6%) could be mapped to orphan Biosynthetic Gene Clusters (BGCs) of related plant pathogens belonging to family Botryosphaeriaceae. Also, screening for rotenoids biosynthetic enzymes led to characterization of a putative chalcone isomerase-like (CHI-like) protein. This is the first report of rotenoids biosynthesized by a fungus, unveiling a unique ability of P. stromaticum.
机译：在本研究中，我们结合液相色谱高分辨率质谱（LC-HRMS）和高通量DNA测序从Pseudofusicoccum stromaticum，关联到药用植物Myracrodruon urundeuva的内生真菌细胞毒性勘探专门代谢物。 LC-HRMS剖析允许识别从P. stromaticum肉汤在乙酸乙酯萃取液推定11的化合物。此外，通过细胞毒性与质谱分析结合引导的化学分馏，得到三种化合物的分离：环肽环 - 升-PHE-d-LEU-1-LEU-1-亮氨酸-1- LLE与已知鱼藤酮类rotenolone和沿灰叶素。 MTT分析表明，灰叶素（IC500.51克毫升（-1））具有强的细胞毒性作用，并且可以指出作为负责P. stromaticum的抗增殖活性的化合物。新一代测序（NGS）和P. stromaticum基因组草图的基因组中挖掘发现56个重叠群编纂专门代谢产物生物合成相关酶。近一半的这些基因（44.6％）的可以映射到孤儿生物合成基因簇的属于家庭Botryosphaeriaceae相关植物病原体（BGCs）。另外，筛选鱼藤酮类生物合成酶导致了推定的查耳酮异构酶表征样（CHI样）蛋白。这是由真菌生物合成鱼藤酮类的第一份报告，揭开P. stromaticum的独特能力。

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