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Synthesis and characterization of green tea (Camellia sinensis (L.) Kuntze) extract and its major components-based nanoflowers: a new strategy to enhance antimicrobial activity

机译:绿茶提取物及其主要成分的纳米花的合成与表征:增强抗菌活性的新策略

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In this study, for the first time, a novel organic–inorganic nanobio-antimicrobial agent called “nanoflowers” (Nfs) from Camellia sinensis (L.) Kuntze extracts and its main components were produced and the increase in the antimicrobial activity of the Nfs was elucidated. While the green tea (GT) extract (obtained in ethanol and water) and its main components (caffeine, catechin) were involved as organic components in the formation of the Nfs, copper(II) ions (Cu2+) were the inorganic component. The structures of the Nfs were examined with several techniques such as Scanning Electron Microscopy (SEM), Fourier transform infrared spectrometry (FT-IR) and Energy-Dispersive X-ray (EDX) spectroscopy. The structural examination demonstrated that the presence of Cu–O and Cu–N bonds in Nfs can be an indication of the Nfs formation. Antimicrobial activities of the free GT extracts, caffeine (cf), catechin (ct) and their Nfs were systematically studied against Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922) and Candida albicans (ATCC 90028) with broth microdilution and short time-kill assay. The peroxidase-mimicking activity depending on a Fenton-like reaction mechanism of the Nfs was measured against guaiacol in the presence of H2O2. In addition, total phenol contents of free GT extracts and their Nfs were calculated by Folin Ciocalteu method. Our results demonstrated that plant extract based Nfs technology is promising and may find potential applications in various scientific and technical fields.
机译:在这项研究中,首次生产了一种新的有机-无机纳米生物抗菌剂,称为(L.)的昆士兰提取物及其主要成分,并增加了其含量。阐明了Nfs的抗菌活性。绿茶(GT)提取物(从乙醇和水中获得)及其主要成分(咖啡因,儿茶素)作为有机成分参与Nfs的形成,而铜( II )离子(Cu 2 + )是无机成分。 Nfs的结构用几种技术检查,例如扫描电子显微镜(SEM),傅立叶变换红外光谱(FT-IR)和能量分散X射线(EDX)光谱。结构检查表明,Nfs中存在Cu–O和Cu–N键可以指示Nfs的形成。系统地研究了游离GT提取物,咖啡因(cf),儿茶素(ct)及其Nfs对金黄色葡萄球菌(ATCC 25923),大肠杆菌(ATCC 25922)的抗菌活性)和白色念珠菌(emcc)(ATCC 90028)进行肉汤微稀释和短时间杀伤试验。在H 2 O 2 。另外,游离GT提取物及其Nf的总酚含量通过Folin Ciocalteu方法计算。我们的结果表明,基于植物提取物的Nfs技术是有前途的,并可能在各种科学和技术领域中找到潜在的应用。

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