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Colorimetric detection of streptomycin in milk based on peroxidase-mimicking catalytic activity of gold nanoparticles

机译：基于金纳米粒子过氧化物酶模拟催化活性的比色法测定牛奶中的链霉素

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A novel colorimetric aptamer sensor (aptasensor) for the detection of streptomycin (STR) is developed based on the peroxidase-like activity of gold nanoparticles (AuNPs) and their interaction with STR-aptamer complex. It is known that AuNPs can easily oxidize substrates in the presence of hydrogen peroxide in a manner identical to peroxidase and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) is a commonly used peroxidase substrate with green oxidation product and a characteristic absorption peak at 733 nm. In the absence of STR, STR1 aptamer is adsorbed on the surface of AuNPs, and restrains its catalytic activity due to shield effect of ssDNA sequence against substrates. STR first binds with STR1 aptamer to form an STR-aptamer complex; thus, aptamer sequences do not get absorbed on the surface of the nanoparticles and their peroxidase activity is further greatly enhanced by the STR-aptamer complex, which enables AuNPs to catalyse the oxidation of the substrates. To achieve a higher level of sensitivity, several key parameters of the proposed aptasensor such as pH, concentrations of STR1 aptamer and AuNPs, and incubation and reaction temperature have been investigated. Under optimal conditions, the proposed aptasensor can detect STR in a linear range from 0.1 μM to 0.5 μM with a limit of detection (LOD) as low as 86 nM and exhibits good selectivity. Moreover, further studies also validate the applicability of the proposed aptasensor in milk samples, revealing that it may have enormous potential utility for practical STR detection in food products in the future.
机译：基于金纳米颗粒（AuNPs）的过氧化物酶样活性及其与STR-适体复合物的相互作用，开发了一种用于检测链霉素（STR）的新型比色适体传感器（aptasensor）。已知AuNPs可以在过氧化氢存在下以与过氧化物酶相同的方式轻易氧化底物，而2,2'-叠氮基双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）是常用的过氧化物酶底物绿色氧化产物，并在733 nm处有一个特征吸收峰。在没有STR的情况下，STR1适配子被吸附在AuNPs的表面上，并由于ssDNA序列对底物的屏蔽作用而抑制了其催化活性。 STR首先与STR1适配子结合形成STR适配子复合体;因此，适体序列不会被吸收在纳米颗粒的表面上，并且它们的过氧化物酶活性被STR-适体复合物进一步增强，这使得AuNPs能够催化底物的氧化。为了获得更高的灵敏度，已对拟议的适体传感器的几个关键参数进行了研究，例如pH，STR1适体和AuNPs的浓度以及孵育和反应温度。在最佳条件下，拟议的适体传感器可在0.1μM至0.5μM的线性范围内检测STR，检测限（LOD）低至86 nM，并具有良好的选择性。此外，进一步的研究也验证了所提出的适体传感器在牛奶样品中的适用性，表明其将来在食品中实际STR检测中可能具有巨大的潜在实用性。

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