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首页> 外文期刊>RSC Advances >CdTe/CdS quantum dot-labeled fluorescent immunochromatography test strips for rapid detection of Escherichia coli O157:H7
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CdTe/CdS quantum dot-labeled fluorescent immunochromatography test strips for rapid detection of Escherichia coli O157:H7

机译：CdTe / CdS量子点标记荧光免疫层析试纸用于快速检测大肠杆菌 O157：H7

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Immunochromatography test strips have been widely used to detect foodborne pathogens due to their high sensitivity, stability and specificity, and the selection of the label is an important factor. In this study, CdTe/CdS quantum dots with a high fluorescence were synthesized as a fluorescence label to conjugate with antibodies to prepare an immunochromatography test strip for the detection of Escherichia coli O157:H7 (E. coli O157:H7). Antibody-quantum dot conjugation was coated onto a glass fiber membrane, which played the role of the conjugate pad. E. coli O157:H7 polyclonal antibodies and goat anti-rabbit immunoglobulin G (Ig G) were used to create the test line and the control line, respectively. The detection limit was determined as 104 CFU mL?1 and no cross-reactions were discovered with other Escherichia coli and other strains. In addition, the strips detected effectively after storing at room temperature in the dark for 35 d. The detection was easy to perform, rapidly and sensitively, and the total detection time was less than 12 min to obtain accurate results, and this method has the potential to be a powerful tool to detect bacteria quantitatively.
机译：免疫层析试纸由于其高灵敏度，稳定性和特异性而被广泛用于检测食源性病原体，标记的选择是一个重要因素。在这项研究中，合成具有高荧光的CdTe / CdS量子点作为荧光标记以与抗体缀合，以制备用于检测大肠杆菌 O157：H7（ E大肠杆菌 O157：H7）。将抗体-量子点缀合涂覆在玻璃纤维膜上，该膜起缀合物垫的作用。 E。使用大肠杆菌O157：H7多克隆抗体和山羊抗兔免疫球蛋白G（Ig G）分别创建测试线和对照线。检出限确定为10 4 CFU mL ？1 ，未发现与其他< em>大肠杆菌和其他菌株。另外，在室温下在黑暗中存储35 d后，这些条带被有效检测。该检测操作简便，快速且灵敏，总检测时间少于12分钟即可获得准确的结果，该方法有可能成为定量检测细菌的有力工具。

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