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首页> 外文期刊>RSC Advances >Exploiting intrinsic fluorescence spectroscopy to discriminate between Acinetobacter calcoaceticus–Acinetobacter baumannii complex species
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Exploiting intrinsic fluorescence spectroscopy to discriminate between Acinetobacter calcoaceticus–Acinetobacter baumannii complex species

机译：利用内在荧光光谱技术来区分钙不动杆菌-鲍曼不动杆菌复杂物种

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摘要

Several spectroscopic techniques, such as infrared, Raman, fluorescence and mass spectrometry, have been tested in the context of bacterial typing. The degree of success is highly dependent on the taxonomic level. Intrinsic fluorescence spectroscopy, detecting natural bacterial fluorophores, has been claimed to be a reliable alternative to the standard typing methods, with some published works reporting its success. In this work we evaluate the ability of this technique to discriminate between four closely related species belonging to the so-called Acinetobacter calcoaceticus–Acinetobacter baumannii complex. Single-point and two-dimensional fluorescence spectral data were acquired at room temperature and 25 °C. Spectra were analysed by partial least squares discriminant analysis and soft independent modelling of class analogy. The percentages of correct species assignments, ranging from 4.2–97.7%, were highly dependent on the experimental conditions and the method of data analysis. It seems that the results benefit from strict temperature control being those achieved with two-dimensional data slightly better than with single-point data. Nevertheless, it was impossible to achieve a satisfactory percentage of correct assignments for the four species simultaneously, pointing to several limitations of this technique for such bacterial species typing purposes.
机译：在细菌分型的背景下，已经测试了几种光谱技术，例如红外，拉曼，荧光和质谱。成功的程度在很大程度上取决于分类标准。检测自然细菌荧光团的内在荧光光谱法被认为是标准打字方法的可靠替代品，一些已发表的著作报告了其成功。在这项工作中，我们评估了该技术区分属于所谓“钙乙酸不动杆菌”-“鲍曼不动杆菌”复合体的四个紧密相关物种的能力。在室温和25°C下获取单点和二维荧光光谱数据。通过偏最小二乘判别分析和类比的软独立建模来分析光谱。正确的物种分配百分比范围为4.2–97.7％，在很大程度上取决于实验条件和数据分析方法。似乎严格的温度控制可以使结果受益，因为二维数据比单点数据要好。然而，不可能同时为这四个物种获得令人满意的正确分配百分比，这表明该技术出于此类细菌物种分型目的的一些局限性。

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