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Identification and characterization of epiphytic yeasts on apples in China

机译：中国苹果附生酵母的鉴定与表征

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Currently, little is known regarding the prevalence and diversity of epiphytic yeasts on apples. Therefore, in this study we attempted to identify and characterize yeasts on apples for their potential cider-making performance. A total of 754 yeast strains were isolated from the skins of apples collected from eight provinces in the two main ecological regions of China, i.e., the Loess Plateau and Bo Hai Gulf regions. More than 71 species belonging to 24 genera were identified and Aureobasidium (A.) pullulans and Hanseniaspora (H.) uvarum were the most predominant yeast species. We did not observe any Saccharomyces cerevisiae strains on the associated apple samples. Correspondence analysis revealed the following connection between yeast genera and the provinces: Hanseniaspora in Shaanxi, Rhodotorula in Liaoning, Candida in Shandong, Aureobasidium in Shanxi and Pichia in Hebei. No dominant genera were observed in apples from Gansu, Henan and Ningxia. Although genetic differences among the 23 H. uvarum strains were identified, there was no clear link between the isolates as to origin, genotype and phenotype following RAPD-PCR analysis and some qualitative evaluation of the yeast biochemical activities.
机译：目前，关于附生酵母在苹果上的流行和多样性知之甚少。因此，在这项研究中，我们试图鉴定和鉴定苹果中潜在的苹果酒生产性能的酵母。从在中国两个主要生态区域，即黄土高原和渤海湾地区的八个省份收集的苹果皮中分离出总共754种酵母菌株。鉴定出属于24个属的71个以上物种，并且 Aureobasidium （ A。） pullulans 和 Hanseniaspora （ H。） uvarum 是最主要的酵母菌种。我们没有在相关的苹果样品上观察到任何酿酒酵母菌株。对应分析表明，酵母属与各省之间存在以下联系：陕西的，辽宁的 Rhodotorula ，山东的 Candida ，金银花在山西和毕赤酵母在河北。在甘肃，河南和宁夏的苹果中没有观察到优势属。虽然在23个 H之间有遗传差异。鉴定出无毒菌株，通过RAPD-PCR分析和酵母生化活性的定性评估，分离株之间在起源，基因型和表型之间没有明确的联系。

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