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首页> 外文期刊>RSC Advances >Digital immunoassay of a prostate-specific antigen using gold nanorods and magnetic nanoparticles
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Digital immunoassay of a prostate-specific antigen using gold nanorods and magnetic nanoparticles

机译：使用金纳米棒和磁性纳米粒子对前列腺特异性抗原进行数字免疫测定

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摘要

Herein, a digital immunoassay was developed by counting highly confined gold nanorod (AuNR) probes with the aid of magnetic nanoparticles (MNPs). MNPs were conjugated with capture antibodies to concentrate the target analytes. AuNRs were conjugated with a mixture of the detection antibody and randomly selected DNA, where the detection antibody was applied to signal the target binding event and DNA was applied to provide sufficient negative charges. The bicomponent AuNR probes in the target-mediated immunocomplexes were eluted and confined onto a positively charged glass slide on the basis of electrostatic attraction for accurate counting. The combination of the magnetic concentration of the target analytes and the confinement of the DNA/antibody AuNR probes results in improved detection efficiency; a good linear range from 0.01 to 10 pg mL?1 and a detection limit of 8 fg mL?1 (～226 aM) were achieved for the detection of prostate-specific antigen (PSA). The proposed method offers the simultaneous advantages of high sensitivity, digital quantification accuracy, lack of requirement for signal or target amplification, wide dose response curve and low detection cost; thus, it shows good potential in practical applications.
机译：本文中，通过借助磁性纳米颗粒（MNP）计数高度受限的金纳米棒（AuNR）探针，开发了一种数字免疫测定法。 MNP与捕获抗体缀合以浓缩目标分析物。将AuNRs与检测抗体和随机选择的DNA的混合物缀合，其中将检测抗体应用于信号靶结合事件，并且将DNA应用于提供足够的负电荷。根据静电吸引，将靶标介导的免疫复合物中的双组分AuNR探针洗脱并限制在带正电的载玻片上，以进行精确计数。目标分析物的磁性浓度与DNA /抗体AuNR探针的限制相结合，可提高检测效率。线性范围从0.01到10 pg mL ？1 ，检测限为8 fg mL ？1 （〜226 aM）用于检测前列腺特异性抗原（PSA）。该方法同时具有灵敏度高，数字定量准确，对信号或靶标扩增的要求低，剂量反应曲线宽，检测成本低等优点。因此，在实际应用中显示出良好的潜力。

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