• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >In vitro and in vivo assessment of nanostructured porous biphasic calcium phosphate ceramics for promoting osteogenesis in an osteoporotic environment
【24h】

In vitro and in vivo assessment of nanostructured porous biphasic calcium phosphate ceramics for promoting osteogenesis in an osteoporotic environment

机译：纳米结构多孔双相磷酸钙陶瓷在骨质疏松环境中促进成骨的体外和体内评估

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Treatment of bone defects in osteoporotic patients with bone substitutes is difficult, due to insufficient osseointegration. The development of appropriate biomaterials to solve the problem requires the assessment of the material performance in an osteoporotic environment, which is rarely investigated. Herein, nanostructured biphasic calcium phosphate (nBCP) ceramics were prepared via the incorporation of hydroxyapatite nanoparticles (HANPs) into porous biphasic CaP (BCP) substrates, leading to an increase of over 500% in the specific surface area. Primary osteoblasts harvested from osteoporotic rats were cultured on the nBCP ceramics, and it was found that the osteoblast functions, including proliferation, alkaline phosphatase activity, osteocalcin secretion and expression of osteogenic genes, were significantly enhanced compared with osteoblasts grown on non-nanostructured BCP ceramics. To further assess the osteoinduction ability, the ceramics were implanted in the femur of osteoporotic rats. Compared to the rats implanted with non-nanostructured BCP ceramics, a higher amount of mechanically matured bone was newly formed in the rats with nBCP ceramics after 6 weeks of implantation. Such enhanced osteoinduction ability of the nBCP ceramics may be due to the incorporated HANPs, as well as the nanostructured topography induced by the HANPs. These results indicate good in vitro and in vivo osteoinductivity of the nBCP ceramics in an osteoporotic environment and offer potential benefits for treating bone defects in osteoporotic patients.
机译：由于骨整合不足，很难用骨替代物治疗骨质疏松患者的骨缺损。为了解决该问题，开发合适的生物材料需要评估在骨质疏松环境中的材料性能，这很少进行研究。在本文中，通过将羟基磷灰石纳米颗粒（HANP）掺入多孔双相CaP（BCP）基底中来制备纳米结构的双相磷酸钙（nBCP）陶瓷，导致比表面积增加了500％以上。从骨质疏松大鼠中收获的原代成骨细胞在nBCP陶瓷上培养，发现与在非纳米结构BCP陶瓷上生长的成骨细胞相比，成骨细胞的功能（包括增殖，碱性磷酸酶活性，骨钙素分泌和成骨基因的表达）得到了显着增强。 。为了进一步评估骨诱导能力，将陶瓷植入骨质疏松大鼠的股骨中。与植入非纳米结构BCP陶瓷的大鼠相比，植入nBCP陶瓷的大鼠在植入6周后，新形成了更高数量的机械成熟骨骼。 nBCP陶瓷的这种增强的骨诱导能力可能归因于掺入的HANP，以及由HANP诱导的纳米结构形貌。这些结果表明，nBCP陶瓷在骨质疏松环境中具有良好的体外和体内骨诱导性，并为治疗骨质疏松患者的骨缺损提供了潜在的益处。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号