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首页> 外文期刊>RSC Advances >Enhancing the osteogenic capacity of MG63 cells through N-isopropylacrylamide-modified polyethylenimine-mediated oligodeoxynucleotide MT01 delivery
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Enhancing the osteogenic capacity of MG63 cells through N-isopropylacrylamide-modified polyethylenimine-mediated oligodeoxynucleotide MT01 delivery

机译：通过 N -异丙基丙烯酰胺修饰的聚乙烯亚胺介导的寡脱氧核苷酸MT01递送增强MG63细胞的成骨能力

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摘要

An oligodeoxynucleotide (ODN), with its design based on human mitochondrial DNA (MT01), has been demonstrated to promote the proliferation and activation of osteoblasts. However, critical issues such as poor oligonucleotide stability in the presence of nucleases currently limit its broad application. This study evaluated whether N-isopropylacrylamide-modified polyethylenimine (PEN) nanoparticles could effectively deliver ODN MT01 locally with low or no toxicity and promote osteoblast differentiation in vitro. Our data demonstrate that at a w/w ratio of 6?:?1, PEN/MT01 nanoparticles were effectively transfected into MG63 cells without cytotoxicity, whereby they promoted cell proliferation in vitro. Quantitative PCR and western blotting analyses showed that the PEN/MT01 complexes enhanced mRNA and protein expression of osteoprotegerin, Sp7, and runt-related transcription factor 2, and decreased levels of receptor activator of nuclear factor kappa-B ligand, which are markers indicative of MG63 differentiation into preosteoblasts or mature osteoblasts. This approach demonstrates the potential of using PEN as a biocompatible gene therapy carrier to address regeneration of bone defects.
机译：寡聚脱氧核苷酸（ODN），其基于人线粒体DNA（MT01）的设计，已被证明可以促进成骨细胞的增殖和活化。但是，诸如核酸酶存在下寡核苷酸稳定性差等关键问题目前限制了其广泛应用。这项研究评估了 N -异丙基丙烯酰胺改性的聚乙烯亚胺（PEN）纳米颗粒能否以低毒性或无毒性的方式有效地局部递送ODN MT01，并促进体外成骨细胞的分化。我们的数据表明，PEN / MT01纳米粒的w / w比率为6？：？1，可以有效地转染MG63细胞而无细胞毒性，从而促进体外细胞增殖。定量PCR和Western印迹分析表明PEN / MT01复合物增强了骨保护素，Sp7和矮子相关转录因子2的mRNA和蛋白表达，并降低了核因子kappa-B配体的受体激活剂水平，这是表明MG63分化为成骨细胞或成熟成骨细胞。这种方法证明了使用PEN作为生物相容性基因治疗载体解决骨缺损再生的潜力。

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