Ion-specificity in protein binding and recovery for the responsive hydrophobic poly(vinylcaprolactam) ligand

Zizhao Liu; S. Ranil Wickramasinghe; Xianghong Qian

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Ion-specificity in protein binding and recovery for the responsive hydrophobic poly(vinylcaprolactam) ligand

机译：响应性疏水性聚己内酰胺配体在蛋白质结合和回收中的离子特异性

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The conformational switch between the hydrophobic state and hydrophilic state of thermo-responsive poly(vinylcaprolactam) (PVCL) has great potential for protein binding and elution applications during protein purification as a hydrophobic interaction chromatography ligand. The lower critical solution temperature (LCST) of PVCL is strongly salt type and salt concentration dependent. Here PVCL polymer chains were grafted on regenerated cellulose membranes using controllable atom-transfer radical polymerization (ATRP) for protein binding/elution studies. Protein binding and recovery demonstrates strong salt type and salt concentration dependency. The effects of salt ions on the static and dynamic binding interactions were elucidated and correlated well with the conformational and hydrophobicity changes of the responsive ligand.

机译：热响应性聚（乙烯基己内酰胺）（PVCL）的疏水状态和亲水状态之间的构象转换具有作为疏水相互作用色谱配体的巨大潜力，可用于蛋白质纯化过程中的蛋白质结合和洗脱应用。 PVCL的较低临界溶液温度（LCST）与盐类型和盐浓度密切相关。在这里，PVCL聚合物链使用可控的原子转移自由基聚合（ATRP）接枝到再生纤维素膜上，用于蛋白质结合/洗脱研究。蛋白质结合和回收显示出强烈的盐类型和盐浓度依赖性。阐明了盐离子对静态和动态结合相互作用的影响，并将其与响应性配体的构象和疏水性变化很好地关联。

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《RSC Advances》 |2017年第58期|共10页
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Zizhao Liu; S. Ranil Wickramasinghe; Xianghong Qian;
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1. Chitosan microparticles embedded with multi-responsive poly(N-vinylcaprolactam-co-itaconic acid-co-ethylene-glycol dimethacrylate)-based hydrogel nanoparticles as a new carrier for delivery of hydrophobic drugs [J] . Fonseca Jessica de Matos, Medeiros Simone de Fatima, Alves Gizelda Maria, Colloids and Surfaces, B. Biointerfaces . 2019,第期

机译：壳聚糖微粒嵌入多响应聚（N-乙烯基己内酰胺 - 共衣酸 - 共乙二醇二甲基丙烯酸酯）的基于水凝胶纳米粒子，作为新的载体，用于疏水药物
2. Stimuli-responsive chitosan-graft-poly(N-vinylcaprolactam) as a promising material for controlled hydrophobic drug delivery [J] . Prabaharan M, Grailer JJ, Steeber DA, Macromolecular bioscience . 2008,第9期

机译：刺激响应的壳聚糖接枝聚（N-乙烯基己内酰胺）作为一种有望用于控制疏水性药物输送的材料
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机译：通过1-苯胺基萘-8-磺酸盐评估，配体结合可诱导叶酸结合蛋白的疏水性急剧下降，从而抑制疏水脱辅基蛋白的自缔合
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机译：疏水相互作用色谱中蛋白质与疏水配体之间的相互作用的微量微量微量微量微量微量微量微量微量微量微量微量微量微量微量化研究：配体链长度，密度和结合蛋白质的影响
5. Biophysical studies of fatty acid binding proteins and hydrophobic ligands. [D] . Kizilbash, Nadeem Abbas. 2003

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7. Ion-specificity in protein binding and recovery for the responsive hydrophobic poly(vinylcaprolactam) ligand [O] . Zizhao Liu, S. Ranil Wickramasinghe, Xianghong Qian 2017

机译：蛋白质特异性蛋白质结合和恢复响应性疏水聚（乙烯基己内酰胺）配体
8. Role of Low Frequency Collective Modes in Biological Function: Ligand Binding and Cooperativity in Calcium-Binding Proteins [R] . Trewhella, J. 2001

机译：低频集体模式在生物功能中的作用：钙结合蛋白的配体结合和协同作用

Ion-specificity in protein binding and recovery for the responsive hydrophobic poly(vinylcaprolactam) ligand

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