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Bio-ethanol production by Zymomonas mobilis using pretreated dairy manure as a carbon and nitrogen source

机译:运动发酵单胞菌使用预处理的乳牛粪作为碳和氮源生产生物乙醇

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Dairy manure contains high levels of cellulose, hemicellulose and a nitrogen source. These properties make dairy manure a potential biomass source for ethanol production. In this study, a dilute NaOH pretreatment and enzymatic hydrolysis were carried out for the degradation of lignocellulose from dairy manure. The response surface method and Box–Behnken design were first applied in order to optimize the NaOH pretreatment. Under the optimization conditions, 21.14 g L?1 of glucose and 9.48 g L?1 of xylose were obtained from continuous enzymolysis. The maximum ethanol concentration achieved reached 10.55 g L?1 when using ethanologenic Zymomonas mobilis without an additional nitrogen source. This produced a 71.91% ethanol yield from manure hydrolysate medium. These results demonstrate the economic benefits of using nitrogen from a dairy manure source for balancing the C/N ratio, without the need for an additional nitrogen source. The results from this study could extend beyond cellulosic ethanol for the production of other chemicals.
机译:乳牛粪中含有大量的纤维素,半纤维素和氮源。这些特性使奶牛粪便成为乙醇生产的潜在生物质来源。在这项研究中,进行了稀NaOH预处理和酶水解,以降解奶牛粪中的木质纤维素。响应面法和Box–Behnken设计首先用于优化NaOH预处理。在优化条件下,获得了21.14 g L ?1 葡萄糖和9.48 g L ?1 木糖来自连续的酶解。当使用不带氮源的产乙醇运动发酵单胞菌时,最大乙醇浓度达到10.55 g L ?1 。这从粪便水解产物培养基产生了71.91%的乙醇产率。这些结果表明,使用奶牛粪便来源的氮来平衡C / N比具有经济效益,而无需额外的氮源。这项研究的结果可能会超出纤维素乙醇生产其他化学品的范围。

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