Selection of aptamers for AMACR detection from DNA libraries with different primers

Deng-Kai Yang; Chia-Fu Chou; Lin-Chi Chen

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Selection of aptamers for AMACR detection from DNA libraries with different primers

机译：从具有不同引物的DNA文库中选择用于AMACR检测的适体

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In this work, we investigated aptamer selection against alpha-methylacyl-CoA racemase (AMACR) with three DNA libraries bearing different primers. Because of increased selection diversity, aptamers with varied structural properties, such as pre-folded, induced-folding, high and low primer-dependent conformations, were discovered. From the selection results, a dimeric aptamer was constructed and capable of detecting over-expression of AMACR in prostate cancer cell lines. In summary, this study demonstrates a library-based approach to obtain aptamers with different binding properties and provides distinct aptamers for flexible design of AMACR detection.

机译：在这项工作中，我们用带有不同引物的三个DNA库研究了针对α-甲基酰基辅酶A消旋酶（AMACR）的适体选择。由于增加的选择多样性，发现了具有不同结构性质的适体，例如预折叠，诱导折叠，高和低的引物依赖性构象。根据选择结果，构建了二聚体适体，其能够检测前列腺癌细胞系中AMACR的过表达。总而言之，这项研究展示了一种基于文库的方法来获得具有不同结合特性的适体，并为AMACR检测的灵活设计提供了独特的适体。

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《RSC Advances》 |2018年第34期|共8页
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Deng-Kai Yang; Chia-Fu Chou; Lin-Chi Chen;
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中图分类化学;
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1. Selection of aptamers for AMACR detection from DNA libraries with different primers [J] . Yang Deng-Kai, Chou Chia-Fu, Chen Lin-Chi RSC Advances . 2018,第34期

机译：从DNA文库中选择Anacr检测与不同引物的适体
2. Generation of Aptamers from A Primer-Free Randomized ssDNA Library Using Magnetic-Assisted Rapid Aptamer Selection [J] . Shih-Ming Tsao, Ji-Ching Lai, Horng-Er Horng, Scientific reports. . 2017,第1期

机译：使用磁辅助快速适体选择从无词底随机SSDNA文库产生适体
3. Minimal primer and primer-free SELEX protocols for selection of aptamers from random DNA libraries. [J] . Pan W, Xin P, Clawson G BioTechniques . 2008,第3期

机译：从随机DNA文库中选择适体的最小引物和无引物SELEX方案。
4. Selection of Aptamer Pairs for Development of High-Affinity Bivalent DNA Aptamers Using Capillary Electrophoresis [C] . Saori Miyauchi, Koji Wakui, Akane Yamaguchi 日本化学会春季年会講演予稿集 . 2018

机译：选择适体对使用毛细管电泳进行高亲和力二价DNA适体的开发
5. Part I. Design and synthesis of prototypes as leads on a compound library aimed at plasmepsin II inhibition. Part II. Synthesis and evaluation of modified nucleotides for DNA aptamer selection [D] . DiDonato, Dana A. 2006

机译：第一部分：原型的设计和合成，作为针对纤溶酶II抑制的化合物库上的先导。第二部分用于DNA适体选择的修饰核苷酸的合成和评估
6. Generation of Aptamers from A Primer-Free Randomized ssDNA Library Using Magnetic-Assisted Rapid Aptamer Selection [O] . Shih-Ming Tsao, Ji-Ching Lai, Horng-Er Horng, -1

机译：使用磁性辅助的快速适体选择从无引物的随机ssDNA文库中生成适体
7. Selection of aptamers for AMACR detection from DNA libraries with different primers [O] . Deng-Kai Yang, Chia-Fu Chou, Lin-Chi Chen 2018

机译：从DNA文库中选择Anacr检测与不同引物的适体
8. Aptamer Selection Express: A Rapid Single-Step Selection of Double Stranded DNA Capture Elements (Briefing Charts). Interim Report [R] . Kiel, J., Fan, M., Holwitt, E., 2009

机译：适体选择快递：双链DNa捕获元件的快速单步选择（简报图）。中期报告

Selection of aptamers for AMACR detection from DNA libraries with different primers

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