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首页> 外文期刊>RSC Advances >Silk fibroin-derived peptide directed silver nanoclusters for cell imaging
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Silk fibroin-derived peptide directed silver nanoclusters for cell imaging

机译：丝素蛋白衍生的肽导向的银纳米簇用于细胞成像

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Fluorescent silver nanoclusters (Ag NCs) that are capable of emitting green light have been synthesized using a peptide derived from the C terminal of silk fibroin heavy chain (CSH) via a one-pot, green, and facile synthesis method. The emission was also found to be stable at the excitation wavelength and the fluorescence quantum yield of Ag NCs was measured to be 1.1%. Matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) indicated the presence of a range of Ag species that correspond to Ag1, Ag2, Ag3 and Ag4. Transmission electron microscopic analyses suggested that the formed particles are uniform and well dispersive with an average diameter of 2.5?nm. The Ag NCs were successfully applied to cell imaging in murine preosteoblast MC3T3-E1 cells. Finally, Ag NCs observed by MTT exhibited distinct cytotoxicity at CSH–Ag NCs concentrations of 600 μM. Based on the concept of utilizing a functional peptide from nature, this study demonstrates a novel approach to fabricate aqueous metal nanoclusters for tracking applications in bioimaging.
机译：经由一锅，绿色且简便的合成方法，使用衍生自丝素蛋白重链（CSH）C末端的肽合成了能够发射绿光的荧光银纳米簇（Ag NCs）。还发现发射在激发波长处是稳定的，并且测得的Ag NCs的荧光量子产率为1.1％。基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）表明存在一系列与Ag1，Ag2，Ag3和Ag4对应的Ag物质。透射电子显微镜分析表明，所形成的颗粒是均匀且分散良好的，平均直径为2.5？nm。 Ag NCs已成功应用于小鼠成骨细胞MC3T3-E1细胞的细胞成像。最后，MTT观察到的Ag NCs在CSH–Ag NCs浓度为600μM时表现出明显的细胞毒性。基于利用来自自然界的功能性肽的概念，这项研究表明了一种新颖的方法来制造水性金属纳米簇，以追踪生物成像中的应用。

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