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Understanding the interactability of chikungunya virus proteins via molecular recognition feature analysis

机译：通过分子识别特征分析了解基孔肯雅病毒蛋白的相互作用

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The chikungunya virus (CHIKV) is an alphavirus that has an enveloped icosahedral capsid and is transmitted by Aedes sp. mosquitos. It contains four non-structural proteins, namely nsP1, nsP2, nsP3, and nsP4, encoded at the 5′ end of the genome, and five structural proteins encoded at the 3′ end of the genome, including three glycosylated proteins, namely E1, E2, E3, a small 64 amino-acids glycoprotein 6K, and one non-glycosylated nucleocapsid protein C. The surface of this positive-stranded RNA alphavirus is covered with 80 trimeric glycoprotein spikes, which facilitate viral access into the host cell, with each consisting of three copies of E1-E2 heterodimers. The proper folding of p62, which is the precursor of E2, and formation of the E1-p62 heterodimers are controlled by E3, which is therefore essential for producing mature spikes on the alphavirus surface. Finally, 6K, a small 64 amino-acids glycoprotein, assists in the translocation of structural polyproteins to the endoplasmic reticulum and in the cleavage of p62 into mature structural proteins E2. The CHIKV proteins have been shown to contain variable levels of intrinsic disorder, often containing intrinsically disordered protein regions (IDPRs). IDPRs can interact with many unrelated partners, and these interactions are frequently accompanied by a transition from a disordered to ordered state. The corresponding sub-regions of IDPRs are acknowledged as molecular recognition features (MoRFs). Although the existence of IDPRs in CHIKV proteome has been analyzed, the prevalence of disorder-based protein–protein interactions ( i.e. MoRF) in this virus have not been evaluated as of yet. To fill this gap, in our study, we utilized several computational methods to identify the MoRFs regions in CHIKV proteins. These computational tools included ANCHOR, DISOPRED3, MoRFpred and MoRFchibi_web server. These analyses revealed the presence of numerous MoRF regions in all the CHIKV proteins. In future, the results of this study could be used to identify the nature of chikungunya virus pathogenesis and might be helpful in designing drugs against this virus.
机译：基孔肯雅热病毒（CHIKV）是一种具有包膜二十面体衣壳的甲型病毒，由伊蚊（Aedes sp。）传播。蚊子。它包含在基因组5'端编码的四个非结构蛋白nsP1，nsP2，nsP3和nsP4，以及在基因组3'端编码的五个结构蛋白，包括三个糖基化蛋白E1， E2，E3，一个小的64个氨基酸的糖蛋白6K和一个非糖基化的核衣壳蛋白C。这种正链RNAα病毒的表面覆盖有80个三聚体糖蛋白刺突，每个刺突均有助于病毒进入宿主细胞由三份E1-E2异二聚体组成。作为E2的前体的p62的正确折叠以及E1-p62异二聚体的形成受E3的控制，因此，这对于在alphavirus表面产生成熟的刺突至关重要。最后，6K是一种64个氨基酸的小糖蛋白，有助于结构多蛋白向内质网的转运，以及将p62裂解为成熟的结构蛋白E2。已经显示CHIKV蛋白包含可变水平的内在障碍，通常包含内在无序的蛋白区域（IDPR）。 IDPR可以与许多无关的伙伴进行交互，而这些交互通常伴随着从无序状态到有序状态的转换。 IDPR的相应子区域被认为是分子识别特征（MoRF）。尽管已经分析了CHIKV蛋白质组中IDPR的存在，但尚未评估该病毒中基于疾病的蛋白质-蛋白质相互作用（即MoRF）的患病率。为了填补这一空白，在我们的研究中，我们使用了几种计算方法来识别CHIKV蛋白中的MoRFs区域。这些计算工具包括ANCHOR，DISOPRED3，MoRFpred和MoRFchibi_web服务器。这些分析揭示了在所有CHIKV蛋白中存在大量MoRF区域。将来，这项研究的结果可用于确定基孔肯雅病毒发病机理的性质，并可能有助于设计针对该病毒的药物。

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