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首页> 外文期刊>RSC Advances >Artificial simulation of salivary and gastrointestinal digestion, and fermentation by human fecal microbiota, of polysaccharides from Dendrobium aphyllum
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Artificial simulation of salivary and gastrointestinal digestion, and fermentation by human fecal microbiota, of polysaccharides from Dendrobium aphyllum

机译：人工模拟石phyl石poly多糖的唾液和胃肠道消化以及人类粪便微生物群的发酵

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In vitro salivary and gastrointestinal (GI) digestion and fermentation of polysaccharides extracted from Dendrobium aphyllum were investigated in this study. Salivary amylase showed no influence on D. aphyllum polysaccharides (DAP). The molecular weight of DAP decreased dramatically during the first 0.5 h of gastric digestion, and then reduced steadily during the subsequent GI tract consumption. The content of reducing sugars increased steadily during GI digestion. Only released free mannose of DAP was detectable by gas chromatography-mass spectrometry analysis during the first 12 h of fermentation, which was contributed by fecal microbiota metabolism. In terms of the fermentation pattern, the pH dropped significantly due to the formation of six types of short-chain fatty acids (SCFAs). This study demonstrates that polysaccharides extracted from D. aphyllum can be digested by the GI tract and are physiologically active in the human large bowel by lowering the pH of the large intestinal environment and promoting the production of SCFAs.
机译：本研究研究了从石end中提取的多糖在体外唾液和胃肠道的消化和发酵。唾液淀粉酶对D.aphyllum多糖（DAP）没有影响。 DAP的分子量在胃消化的最初0.5小时内急剧下降，然后在随后的胃肠道消耗过程中稳定下降。胃肠道消化过程中还原糖的含量稳定增加。在发酵的前12小时内，通过气相色谱-质谱分析只能检测到DAP释放的游离甘露糖，这是由粪便微生物群代谢所致。就发酵模式而言，由于六种类型的短链脂肪酸（SCFA）的形成，pH值明显下降。这项研究表明，通过降低大肠环境的pH值并促进SCFA的产生，从D. aphyllum提取的多糖可以被GI消化，并在人的大肠中具有生理活性。

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