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Antioxidant and anti-inflammatory effects of polyphenols extracted from Ilex latifolia Thunb

机译：冬青叶多酚提取物的抗氧化和抗炎作用

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摘要

To promote the rational and effective application of Ilex latifolia Thunb., a Chinese bitter tea widely consumed as a health beverage, polyphenols were extracted from its leaves and their cellular antioxidant activity (CAA) and anti-inflammatory effect against mouse macrophage RAW 264.7 cells were analyzed. Results showed that the antioxidant capacity of polyphenols was high, and their CAA values in PBS wash and no PBS wash protocols were 6871.42 ± 85.56 and 25161.61 ± 583.55 μmol QE (quercetin equivalents)/100 g phenolic extracts, respectively. In addition, polyphenols from I. latifolia displayed strong inhibition on LPS-induced NO-production in RAW 264.7 cells. Polyphenol treatment inhibited the release of pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) induced by LPS in a dose-dependent manner by ELISA and mRNA expression analysis. Western blot results showed that the anti-inflammatory activity of polyphenols from I. latifolia might be exerted through inhibiting the activation of MAPKs (ERK and JNK) and NF-κB to decrease NO, COX-2 and pro-inflammatory cytokines production. Thus, the polyphenol enriched extracts from I. latifolia are a good source of natural antioxidants with a beneficial effect against inflammation, and they may be applied as a food supplement and/or functional ingredient.
机译：为了促进合理有效地广泛使用作为保健饮料的中国苦茶冬青叶茶，从其叶中提取多酚，并从其细胞抗氧化活性（CAA）和对小鼠巨噬细胞RAW 264.7细胞的抗炎作用中提取了多酚。分析。结果表明，多酚的抗氧化能力很高，在PBS洗涤和无PBS洗涤方案中的CAA值分别为100 g酚提取物6871.42±85.56和25161.61±583.55μmolQE（槲皮素当量）。另外，来自宽叶木槿的多酚对LPS诱导的RAW 264.7细胞中的NO产生强烈抑制作用。通过ELISA和mRNA表达分析，多酚处理以剂量依赖的方式抑制了LPS诱导的促炎性细胞因子（TNF-α，IL-1β和IL-6）的释放。蛋白质印迹结果表明，la叶多酚的抗炎活性可能是通过抑制MAPKs（ERK和JNK）和NF-κB的活化来降低NO，COX-2和促炎细胞因子的产生而发挥的。因此，来自阔叶番荔枝的富含多酚的提取物是天然抗氧化剂的良好来源，具有抗发炎的有益作用，并且它们可以用作食品补充剂和/或功能成分。

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