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Rapid and visual readout of vitamin B1 based on the electrostatic interaction induced aggregation of gold nanoparticles

机译：基于静电相互作用诱导金纳米粒子聚集的维生素B1的快速和视觉读出

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In this work, a simple and rapid colorimetric assay for the quantitative detection of vitamin B1 (VB1) has been fabricated based on citrate-stabilized gold nanoparticles (AuNPs). The UV-Vis spectra of AuNPs varied and the relative color changed from red to purple with the sequential addition of VB1. The characterization results of AuNPs with and without the addition of VB1 confirmed that the observed phenomena were attributed to the aggregation of AuNPs induced by VB1 through electrostatic interaction. The assay was rapid and sensitive to VB1 with a detection limit of 10.9 nM ranging from 30 nM to 650 nM in 15 min. Meanwhile, the developed assay displayed excellent selectivity to VB1 since AuNPs showed negligible response to common metal ions and biological molecules. Moreover, the feasibility for the quantitative detection of VB1 in tablets and human urine samples has also been demonstrated.
机译：在这项工作中，已经基于柠檬酸盐稳定的金纳米颗粒（AuNPs）制备了用于定量检测维生素B1（VB1）的简单快速的比色测定法。依次添加VB1，AuNP的UV-Vis光谱发生变化，相对颜色从红色变为紫色。添加和不添加VB1的AuNPs的表征结果证实，观察到的现象归因于VB1通过静电相互作用诱导的AuNPs的聚集。该测定快速且对VB1敏感，在15分钟内的检出限为10.9 nM，范围为30 nM至650 nM。同时，由于AuNPs对常见金属离子和生物分子的响应可忽略不计，因此开发的测定法对VB1具有极好的选择性。此外，还证明了定量检测片剂和人尿液样品中VB1的可行性。

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