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Rapid and visual readout of vitamin B1 based on the electrostatic interaction induced aggregation of gold nanoparticles

机译：基于静电相互作用诱导金纳米粒子的聚集的维生素B1的快速和视觉读出

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摘要

In this work, a simple and rapid colorimetric assay for the quantitative detection of vitamin B1 (VB1) has been fabricated based on citrate-stabilized gold nanoparticles (AuNPs). The UV-Vis spectra of AuNPs varied and the relative color changed from red to purple with the sequential addition of VB1. The characterization results of AuNPs with and without the addition of VB1 confirmed that the observed phenomena were attributed to the aggregation of AuNPs induced by VB1 through electrostatic interaction. The assay was rapid and sensitive to VB1 with a detection limit of 10.9 nM ranging from 30 nM to 650 nM in 15 min. Meanwhile, the developed assay displayed excellent selectivity to VB1 since AuNPs showed negligible response to common metal ions and biological molecules. Moreover, the feasibility for the quantitative detection of VB1 in tablets and human urine samples has also been demonstrated.
机译：在这项工作中，基于柠檬酸盐稳定的金纳米颗粒（AUNP），制造了用于定量检测维生素B1（VB1）的简单且快速的比色测定。 AUNP的UV-VIS谱不同，相对颜色从红色变为紫色，顺序添加VB1。 AuNP的表征结果具有和不添加VB1，证实观察到的现象归因于VB1通过静电相互作用诱导的AUNP的聚集。 测定对VB1快速且敏感，检测限为10.9nm，在15分钟内为30nm至650nm。 同时，发达的测定显示出对VB1的优异选择性，因为AUNPS显示常见金属离子和生物分子的响应可忽略不计。 此外，还证实了片剂中VB1和人尿液样品的定量检测的可行性。

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  • 来源
    《RSC Advances》 |2018年第62期|共5页
  • 作者

    Lin Liping; Wang Jiajing; Liu Wei; Luo Yaxin; Xiao Yanling; Wang Yuhan;

  • 作者单位

    Fujian Agr &

    Forestry Univ Coll Life Sci Dept Appl Chem Fuzhou 350002 Fujian Peoples R China;

    Fujian Agr &

    Forestry Univ Coll Life Sci Dept Appl Chem Fuzhou 350002 Fujian Peoples R China;

    Fujian Agr &

    Forestry Univ Coll Life Sci Dept Bioinformat Fuzhou 350002 Fujian Peoples R China;

    Fujian Agr &

    Forestry Univ Coll Life Sci Dept Appl Chem Fuzhou 350002 Fujian Peoples R China;

    Fujian Agr &

    Forestry Univ Coll Life Sci Dept Appl Chem Fuzhou 350002 Fujian Peoples R China;

    Fujian Agr &

    Forestry Univ Coll Life Sci Dept Appl Chem Fuzhou 350002 Fujian Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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