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首页> 外文期刊>RSC Advances >Various antibacterial mechanisms of biosynthesized copper oxide nanoparticles against soilborne Ralstonia solanacearum
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Various antibacterial mechanisms of biosynthesized copper oxide nanoparticles against soilborne Ralstonia solanacearum

机译：生物合成的氧化铜纳米粒子对土壤传播的青枯菌的多种抗菌机制

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The substantial antimicrobial efficacy of nanoparticles against phytopathogens has been extensively investigated for advanced agricultural applications. However, few reports have focused on soilborne pathogenic bacteria. The aim of this study was to obtain sustainably synthesized copper oxide nanoparticles (CuONPs) using papaya leaf extracts and investigate the bactericidal activity of these CuONPs against Ralstonia solanacearum , the cause of bacterial wilt, under laboratory and greenhouse conditions. The results showed that CuONPs possessed strong antibacterial activity and that all R. solanacearum were killed after exposure to 250 μg mL ~(?1) CuONPs. CuONPs could interact with bacterial cells to prevent biofilm formation, reduce swarming motility and disturb ATP production. Ultrastructural observations by transmission electron microscopy (TEM) revealed that after interactions with CuONPs, bacterial cells suffered significantly from nanomechanical damage to the cytomembrane, accompanied by the absorption of multiple nanoparticles. In addition, molecular studies identified the downregulation mechanism of a series of genes involving pathogenesis and motility. The control efficiency of CuONPs in tobacco bacterial wilt disease management under greenhouse conditions was verified by root irrigation application, demonstrating that as-prepared CuONPs significantly reduced the disease occurrence and disease index. Our studies focused on developing biosynthesized nanoparticles as a biocompatible alternative for soilborne disease management.
机译：纳米粒子对植物病原体的实质性抗菌功效已被广泛研究用于先进的农业应用。但是，很少有报道关注土壤传播的致病细菌。这项研究的目的是使用番木瓜叶提取物获得可持续合成的氧化铜纳米颗粒（CuONP），并研究这些CuONP在实验室和温室条件下对青枯菌（Ralstonia solanacearum）的杀菌活性。结果表明，CuONPs具有很强的抗菌活性，所有青枯菌在暴露于250μgmL〜（？1）CuONPs后均被杀死。 CuONP可以与细菌细胞相互作用，以防止生物膜形成，减少蜂群运动并干扰ATP的产生。透射电子显微镜（TEM）的超微结构观察表明，与CuONPs相互作用后，细菌细胞受到细胞膜的纳米机械损伤，并伴随着多个纳米颗粒的吸收。此外，分子研究确定了一系列涉及发病机理和运动性的基因的下调机制。通过根系灌溉技术验证了CuONPs在温室条件下控制烟草枯萎病的控制效率，证明了所制备的CuONPs显着减少了疾病的发生和疾病指数。我们的研究致力于开发生物合成的纳米颗粒，作为土壤传播疾病管理的生物相容性替代品。

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