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首页> 外文期刊>RSC Advances >Dual-response detection of Ni2+ and Cu2+ ions by a pyrazolopyrimidine-based fluorescent sensor and the application of this sensor in bioimaging
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Dual-response detection of Ni2+ and Cu2+ ions by a pyrazolopyrimidine-based fluorescent sensor and the application of this sensor in bioimaging

机译：吡唑并嘧啶基荧光传感器对Ni2 +和Cu2 +离子的双响应检测及其在生物成像中的应用

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Herein, a dual-response fluorescent sensor, L , based on pyrazolopyrimidine was designed and developed for the simultaneous detection of Ni ~(2+) and Cu ~(2+) ions in the presence of other metal ions; the structural characterization of L was carried out by FTIR spectroscopy, NMR spectroscopy, HRMS and X-ray diffraction analysis. The sensor L effectively displayed fluorescence quenching towards the Ni ~(2+) and Cu ~(2+) ions with high sensitivity without interference from other metal ions. The results reveal that L binds to Ni ~(2+) and Cu ~(2+) in a 2?:?1 pattern, which matches well with the result of the Job's plot. The association constants of L with Ni ~(2+) and Cu ~(2+) were 3.2 × 10 ~(4) M ~(?1) and 7.57 × 10 ~(4) M ~(?1) , respectively. The detection limits (DLs) are down to 8.9 nM for Ni ~(2+) and 8.7 nM for Cu ~(2+) . The fluorescence imaging of L in T-24 cells was investigated because of the low cytotoxicity of L , indicating that L could be used to detect Ni ~(2+) and Cu ~(2+) in living cells.
机译：本文设计并开发了一种基于吡唑并嘧啶的双响应荧光传感器L，用于在其他金属离子存在下同时检测Ni〜（2+）和Cu〜（2+）离子。 L的结构表征通过FTIR光谱，NMR光谱，HRMS和X射线衍射分析进行。传感器L有效地显示了对Ni〜（2+）和Cu〜（2+）离子的荧光猝灭，并且灵敏度很高，而不受其他金属离子的干扰。结果表明，L以2？：？1的模式与Ni〜（2+）和Cu〜（2+）结合，这与乔布斯图的结果非常吻合。 L与Ni〜（2+）和Cu〜（2+）的缔合常数分别为3.2×10〜（4）M〜（？1）和7.57×10〜（4）M〜（？1）。 Ni〜（2+）的检测限（DLs）降至8.9 nM，Cu〜（2+）的检测限降至8.7 nM。由于L的低细胞毒性，研究了L在T-24细胞中的荧光成像，表明L可用于检测活细胞中的Ni〜（2+）和Cu〜（2+）。

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