Dansyl acetyl trehalose: a novel tool to investigate the cellular fate of trehalose

Alessandra Locatelli; Luisa Iommarini; Alessandra Graziadio; Alberto Leoni; Anna Maria Porcelli; Stefano Iotti; Emil Malucelli; Francesco Francia; Giovanni Venturoli; Giovanna Farruggia

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Dansyl acetyl trehalose: a novel tool to investigate the cellular fate of trehalose

机译：丹酰基乙酰海藻糖：研究海藻糖细胞命运的新工具

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A fluorescent derivative of trehalose with two dansyl groups (DAT) has been synthesized. It is characterised by a large Stokes shift, good permeability in human living cells and a well detectable fluorescent signal within the cells. Notably, in intestinal cells DAT is sequestered in vesicles induced by trehalose pre-treatment and colocalizes with lipid droplets.

机译：合成了具有两个丹磺酰基的海藻糖荧光衍生物（DAT）。它的特点是斯托克斯频移大，在人类活细胞中具有良好的渗透性，并且在细胞内具有良好的可检测荧光信号。值得注意的是，在肠道细胞中，DAT被隔离在海藻糖预处理诱导的囊泡中，并与脂质液滴共定位。

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《RSC Advances》 |2019年第27期|共7页
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Alessandra Locatelli; Luisa Iommarini; Alessandra Graziadio; Alberto Leoni; Anna Maria Porcelli; Stefano Iotti; Emil Malucelli; Francesco Francia; Giovanni Venturoli; Giovanna Farruggia;
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1. Dansyl acetyl trehalose: a novel tool to investigate the cellular fate of trehalose [J] . Locatelli Alessandra, Iommarini Luisa, Graziadio Alessandra, RSC Advances . 2019,第27期

机译：乙酰乙酰丙基海藻糖：一种研究海藻糖的细胞命运的新型工具
2. Investigating trehalose synthesis genes after cold acclimation in the Antarctic nematode Panagrolaimus sp. DAW1 Investigating trehalose synthesis genes after cold acclimation in the Antarctic nematode Panagrolaimus sp. DAW1 Investigating trehalose synthesis genes after cold acclimation in the Antarctic nematode Panagrolaimus sp. DAW1 [J] . David A. Wharton, Anna C. Seybold, Michael A. S. Thorne, Biology Open . 2017,第12期

机译：冷适应后在南极线虫Panagrolaimus sp。中调查海藻糖合成基因。 DAW1调查南极线虫Panagrolaimus sp。中冷驯化后的海藻糖合成基因。 DAW1调查南极线虫Panagrolaimus sp。中冷驯化后的海藻糖合成基因。 DAW1
3. Changes in the Amount of Cellular Trehalose, the Activity of Maltooligosyl Trehalose Hydrolase, and the Expression of Its Gene in Response to Salt Stress in the Cyanobacterium Spirulina platensis [J] . Microbes and Environments . 2009,第1期

机译：螺旋藻蓝藻中细胞海藻糖含量的变化，麦芽寡糖基海藻糖水解酶的活性及其基因表达对盐胁迫的响应
4. High Efficiency Expression of Trehalose Synthase in Escherichia coli and Its Use in the Production of Trehalose [C] . Hong-Ling Liu, Rui-Ming Wang, Teng-Fei Wang International conference on applied biotechnology . 2018

机译：海藻糖合酶在大肠杆菌中的高效表达及其在海藻糖生产中的应用
5. Roles of Trehalose and Trehalose-6-Phosphate Synthase in Oxidative Stress Response of Fusarium verticillioides [D] . Oberlie, Nicole R. 2020

机译：海藻糖和海藻糖-6-磷酸合成酶在镰刀镰刀菌氧化胁迫响应中的作用
6. A Novel Member of the Trehalose Transporter Family Functions as an H+-Dependent Trehalose Transporter in the Reabsorption of Trehalose in Malpighian Tubules [O] . Shingo Kikuta, Yuka Hagiwara-Komoda, Hiroaki Noda, 2012

机译：海藻糖转运蛋白家族的新成员在马尔皮基小管中的海藻糖重吸收中起着H +依赖性海藻糖转运蛋白的作用。
7. Changes in the Amount of Cellular Trehalose, the Activity of Maltooligosyl Trehalose Hydrolase, and the Expression of Its Gene in Response to Salt Stress in the Cyanobacterium Spirulina platensis [O] . Kazuko Ohmori, Shigeki Ehira, Satoshi Kimura, 2009

机译：细胞海藻糖量，乳菌酚烯基海藻糖水解酶的活性的变化，以及其基因响应盐胁迫的基因的表达螺旋藻铂金菌

Dansyl acetyl trehalose: a novel tool to investigate the cellular fate of trehalose

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