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首页> 外文期刊>RSC Advances >Biocompatible quantum dot-antibody conjugate for cell imaging, targeting and fluorometric immunoassay: crosslinking, characterization and applications
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Biocompatible quantum dot-antibody conjugate for cell imaging, targeting and fluorometric immunoassay: crosslinking, characterization and applications

机译：用于细胞成像，靶向和荧光免疫分析的生物相容性量子点抗体偶联物：交联，表征和应用

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Quantum dots (QDs) are important fluorescent probes that offer great promise for bio-imaging research due to their superior optical properties. However, QDs for live cell imaging and the tracking of cells need more investigation to simplify processing procedures, improving labeling efficiency, and reducing chronic toxicity. In this study, QDs were functionalized with bovine serum albumin (BSA) via a chemical linker. Anti-human immunoglobulin antibodies were oxidized by sodium periodate to create reactive aldehyde groups for a spontaneous reaction with the amine groups of BSA-modified QDs. An antibody-labeled QD bioconjugate was characterized using agarose gel electrophoresis, dynamic light scattering, and zeta potential. Using fluorescence spectroscopy, we found that the fluorescence of QDs was retained after multiple conjugation steps. The cell-labeling function of the QD bioconjugate was confirmed using an image analyzer and confocal microscopy. The QD bioconjugate specifically targeted human immunoglobulin on the membrane surface of recombinant cells. In addition, the QD bioconjugate applied in fluorometric immunoassay was effective for the quantitative analysis of human immunoglobulin in an enzyme-linked immunosorbent assay. The developed QD bioconjugate may offer a promising platform to develop biocompatible tools to label cells and quantify antibodies in the immunoassay.
机译：量子点（QD）是重要的荧光探针，由于其卓越的光学性能，为生物成像研究提供了广阔的前景。然而，用于活细胞成像和细胞追踪的量子点需要更多的研究，以简化处理程序，提高标记效率并减少慢性毒性。在这项研究中，QDs通过化学接头与牛血清白蛋白（BSA）功能化。抗人免疫球蛋白抗体被高碘酸钠氧化，生成反应性醛基，与BSA修饰QD的胺基自发反应。使用琼脂糖凝胶电泳，动态光散射和Zeta电位对抗体标记的QD生物缀合物进行了表征。使用荧光光谱，我们发现在多个共轭步骤后，量子点的荧光得以保留。使用图像分析仪和共聚焦显微镜确认了QD生物缀合物的细胞标记功能。 QD生物缀合物将人免疫球蛋白特异性靶向重组细胞的膜表面。另外，在荧光免疫测定中应用的QD生物共轭物在酶联免疫吸附测定中对人免疫球蛋白的定量分析有效。研发的QD生物共轭物可能为开发生物相容性工具提供一个有前途的平台，以在免疫测定中标记细胞并定量抗体。

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