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首页> 外文期刊>RSC Advances >Self-organized nanocrystal rings formed by microemulsion for selective recognition of proteins and immunoassays
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Self-organized nanocrystal rings formed by microemulsion for selective recognition of proteins and immunoassays

机译：由微乳液形成的自组织纳米晶体环，用于蛋白质的选择性识别和免疫测定

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A simple and cheap method to fabricate a nanocrystal ring pattern was developed by utilization of a microemulsion in this study. The mixture of polystyrene and stabilizer dichloromethane solution that contained nanocrystal aqueous solution, prepared through shaking, was applied to fabricate a reverse microemulsion. After spreading and evaporating the solvent of microemulsion on a glass slide, an ordered honeycomb film was produced, accompanied by the formation of a nanocrystal ring pattern. The nanocrystal pattern could be readily applied for immunoassays and recognition of proteins. The pattern with antibody marked by a green colored nanocrystal specifically bound with antigen labeled by a red colored nanocrystal, leading to the enhancement in red fluorescent ring pattern and decrease in green fluorescent pattern. When the unlabeled antigen was added, the green fluorescent pattern was recovered. In addition, the ring pattern with immunocomplex could selectively recognize antigen and transferrin proteins. This strategy reveals that these patterns have potential applications in biochips, biosensors, imaging analysis and so forth.
机译：在这项研究中，利用微乳液开发了一种简单且便宜的方法来制造纳米晶体环形图案。施加通过摇动制备的包含纳米晶体水溶液的聚苯乙烯和稳定剂二氯甲烷溶液的混合物，以制备反向微乳液。将微乳液的溶剂铺展并蒸发在载玻片上后，制得有序的蜂窝膜，并形成了纳米晶环形图案。纳米晶体图案可以容易地应用于免疫测定和蛋白质识别。具有由绿色纳米晶体标记的抗体的图案与由红色纳米晶体标记的抗原特异性结合，从而导致红色荧光环图案的增强和绿色荧光图案的减少。当添加未标记的抗原时，恢复了绿色荧光图案。另外，具有免疫复合物的环型可以选择性地识别抗原和转铁蛋白。该策略表明这些模式在生物芯片，生物传感器，成像分析等方面具有潜在的应用。

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